ArticleGut2006
Hepatic stellate cells express synemin, a protein bridging intermediate filaments to focal adhesions.
Article in Gut, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- Characterization of focal adhesion proteins in rodent hepatic stellate cells.Histochemistry and cell biology · 2022Article
- Pancreatic stellate cells - rising stars in pancreatic pathologies.Physiological research · 2021Review
- Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in AβCell death discovery · 2021Article
- LncRNA ANRIL acts as a modular scaffold of WDR5 and HDAC3 complexes and promotes alteration of the vascular smooth muscle cell phenotype.Cell death & disease · 2020Article
- ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5.Frontiers in cell and developmental biology · 2020Article
- Synemin Redefined: Multiple Binding Partners Results in Multifunctionality.Frontiers in cell and developmental biology · 2020Review
- LncRNA GLCC1 promotes colorectal carcinogenesis and glucose metabolism by stabilizing c-Myc.Nature communications · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Identification of markers for quiescent pancreatic stellate cells in the normal human pancreas.Histochemistry and cell biology · 2017Article
- Vimentin knockout results in increased expression of sub-endothelial basement membrane components and carotid stiffness in mice.Scientific reports · 2017Article
- Salvianolic acid B-induced microRNA-152 inhibits liver fibrosis by attenuating DNMT1-mediated Patched1 methylation.Journal of cellular and molecular medicine · 2015Article
- Cytoglobin is expressed in hepatic stellate cells, but not in myofibroblasts, in normal and fibrotic human liver.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2.PloS one · 2014Article
- Syncoilin is an intermediate filament protein in activated hepatic stellate cells.Histochemistry and cell biology · 2014Article
- Synemin isoforms differentially organize cell junctions and desmin filaments in neonatal cardiomyocytes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Stellate cells from rat pancreas are stem cells and can contribute to liver regeneration.PloS one · 2012Article
- Hepatic stellate cells and astrocytes: Stars of scar formation and tissue repair.Cell cycle (Georgetown, Tex.) · 2011Review
- Article
- Blebbistatin inhibits contraction and accelerates migration in mouse hepatic stellate cells.British journal of pharmacology · 2010Article
Corrections and comments
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Authors and funding
11 authors at 8 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsIn the liver, stellate cells play several important (patho)physiological roles. They express a broad but variable spectrum of intermediate filament (IF) proteins. The aim of this study was to investigate the expression and functions of the intermediate filament protein synemin in hepatic stellate cells (HSCs).
methodsIn isolated and cultured rat HSCs, synemin expression was examined by quantitative reverse transcriptase polymerase chain reaction, western blotting, and immunocytochemistry. Protein-protein interaction between synemin and possible binding partners was investigated by co-immunoprecipitation and confocal microscopy.
resultsExpression of synemin was significantly downregulated with increased culture time. In 1-day cultured HSCs, synemin associated with other IF proteins (GFAP, desmin, and vimentin), and with the focal adhesion proteins vinculin and talin, but not with alpha-actinin or paxillin. Synemin IF and focal adhesion proteins co-localised in long slender processes, but not in the lamellipodia. In human and rat liver tissue, the presence of synemin was investigated by immunohistochemistry. In normal rat and human livers, synemin immunoreactivity was found in HSCs, smooth muscle cells of hepatic arterioles, and nerve bundles in portal tracts, but not in portal fibroblasts. In CCl4-intoxicated rat livers and in human cirrhotic livers, immunoreactivity for synemin in the parenchymal tissue was decreased. Thus synemin was expressed in quiescent HSCs but not in portal fibroblasts; and synemin expression decreased with HSC activation in vivo during chronic liver damage and with HSC activation in culture.
conclusionsSynemin forms heteropolymeric filaments with type-III IF proteins and acts as a bridging protein between IFs and a specific type of focal adhesions.
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