ArticleJournal of bacteriology2006
Overexpression of tnaC of Escherichia coli inhibits growth by depleting tRNA2Pro availability.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 30 citations in OpenAlex.
- tRNA-Mediated Plasmid Stabilization for Antibiotic-Free Applications inACS synthetic biology · 2026Article
- A mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism.Nature communications · 2025Article
- Evaluating cellular roles and phenotypes associated with trehalose degradation genes in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2024Article
- An exploratory in silico comparison of open-source codon harmonization tools.Microbial cell factories · 2023Article
- Anaerobic Fungal Mevalonate Pathway Genomic Biases Lead to Heterologous Toxicity Underpredicted by Codon Adaptation Indices.Microorganisms · 2021Article
- Review
- The regulatory TnaC nascent peptide preferentially inhibits release factor 2-mediated hydrolysis of peptidyl-tRNA.The Journal of biological chemistry · 2019Article
- Article
- ATGme: Open-source web application for rare codon identification and custom DNA sequence optimization.BMC bioinformatics · 2015Article
- Expression of codon optimized genes in microbial systems: current industrial applications and perspectives.Frontiers in microbiology · 2014Review
- Engineering genes for predictable protein expression.Protein expression and purification · 2012Review
- Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1.Microbial cell factories · 2010Article
- RNase II is important for A-site mRNA cleavage during ribosome pausing.Molecular microbiology · 2009Article
- Small membrane proteins found by comparative genomics and ribosome binding site models.Molecular microbiology · 2008Article
- The ribosome: a metabolite-responsive transcription regulator.Journal of bacteriology · 2008Review
- RNA-based regulation of genes of tryptophan synthesis and degradation, in bacteria.RNA (New York, N.Y.) · 2007Review
- Ribosome recycling factor and release factor 3 action promotes TnaC-peptidyl-tRNA Dropoff and relieves ribosome stalling during tryptophan induction of tna operon expression in Escherichia coli.Journal of bacteriology · 2007Article
- Gene Designer: a synthetic biology tool for constructing artificial DNA segments.BMC bioinformatics · 2006Article
- Changes produced by bound tryptophan in the ribosome peptidyl transferase center in response to TnaC, a nascent leader peptide.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. Induction results from ribosome stalling after translation of tnaC, the coding region for a 24-residue leader peptide. The last sense codon of tnaC, proline codon 24 (CCU), is translated by tRNA(2)(Pro). We analyzed the consequences of overexpression of tnaC from a multicopy plasmid and observed that under inducing conditions more than 60% of the tRNA(2)(Pro) in the cell was sequestered in ribosomes as TnaC-tRNA(2)(Pro). The half-life of this TnaC-tRNA(2)(Pro) was shown to be 10 to 15 min under these conditions. Plasmid-mediated overexpression of tnaC, under inducing conditions, reduced cell growth rate appreciably. Increasing the tRNA(2)(Pro) level relieved this growth inhibition, suggesting that depletion of this tRNA was primarily responsible for the growth rate reduction. Growth inhibition was not relieved by overexpression of tRNA(1)(Pro), a tRNA(Pro) that translates CCG, but not CCU. Replacing the Pro24CCU codon of tnaC by Pro24CCG, a Pro codon translated by tRNA(1)(Pro), also led to growth rate reduction, and this reduction was relieved by overexpression of tRNA(1)(Pro). These findings establish that the growth inhibition caused by tnaC overexpression during induction by tryptophan is primarily a consequence of tRNA(Pro) depletion, resulting from TnaC-tRNA(Pro) retention within stalled, translating ribosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.