ArticleBMC genomics2006
Highly expressed proteins have an increased frequency of alanine in the second amino acid position.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- EngineeredFrontiers in microbiology · 2025Article
- N-terminal alanine-rich (NTAR) sequences drive precise start codon selection resulting in elevated translation of multiple proteins including ERK1/2.Nucleic acids research · 2023Article
- Peptidyl transferase center decompaction and structural constraints during early protein elongation on the ribosome.Scientific reports · 2021Article
- Construction of a genetically modified T7Select phage system to express the antimicrobial peptide 1018.Journal of microbiology (Seoul, Korea) · 2019Article
- Adenine Enrichment at the Fourth CDS Residue in Bacterial Genes Is Consistent with Error Proofing for +1 Frameshifts.Molecular biology and evolution · 2017Article
- Influence of the second amino acid on recombinant protein expression.Protein expression and purification · 2010Article
- Follow the leader: preference for specific amino acids directly following the initial methionine in proteins of different organisms.Genomics, proteomics & bioinformatics · 2010Article
- Profiling of N-acetylated protein termini provides in-depth insights into the N-terminal nature of the proteome.Molecular & cellular proteomics : MCP · 2010Article
- A universal trend of reduced mRNA stability near the translation-initiation site in prokaryotes and eukaryotes.PLoS computational biology · 2010Article
- Shine-Dalgarno interaction prevents incorporation of noncognate amino acids at the codon following the AUG.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Translation initiation region sequence preferences in Escherichia coli.BMC molecular biology · 2007Article
- The +4G site in Kozak consensus is not related to the efficiency of translation initiation.PloS one · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough the sequence requirements for translation initiation regions have been frequently analysed, usually the highly expressed genes are not treated as a separate dataset.
resultsTo investigate this, we analysed the mRNA regions downstream of initiation codons in nine bacteria, three archaea and three unicellular eukaryotes, comparing the dataset of highly expressed genes to the dataset of all genes. In addition to the detailed analysis of the nucleotide and codon frequencies we compared the N-termini of highly expressed proteins to the N-termini of all proteins coded in the genome.
conclusionThe most conserved pattern was observed at the amino acid level: strong alanine over-representation was observed at the second amino acid position of highly expressed proteins. This pattern is well conserved in all three domains of life.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.