Evidence map›Paper›PMID 16482432›Full record

ArticlePlanta2006

Granule-bound starch synthase cDNA in Chlorella kessleri 11 h: cloning and regulation of expression by CO(2) concentration.

Yasunori Oyama, Asako Izumo, Shoko Fujiwara, Takahiro Shimonaga, Yasunori Nakamura, Mikio Tsuzuki

Abstract read
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In one paragraph

Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 18 citations in OpenAlex.

  1. Acetate Combined with COFoods (Basel, Switzerland) · 2025
    Article
  2. Application of an in situ COBiotechnology for biofuels · 2019
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yasunori OyamaSchool of Life Science, Tokyo University of Pharmacy and Life Science, 192-0392 Horinouchi, Hachioji, Tokyo, Japan.
Asako Izumo
Shoko Fujiwara
Takahiro Shimonaga
Yasunori Nakamura
Mikio Tsuzuki
Tokyo University of Pharmacy and Life Sciences · JPJapan Science and Technology Agency · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cDNA for the granule-bound starch synthase (GBSS; ADP-glucose-starch glucosyltransferase, EC 2.4.1.21) of Chlorella kessleri 11 h was isolated and characterized. CkGBSS encodes a 609-amino acid polypeptide (65,627 Da) that includes an N-terminal hydrophobic signal peptide of 55 amino acids. The deduced amino acid sequence of the mature CkGBSS polypeptide shares a greater identity (65%) to that of the GBSS protein of Chlamydomonas reinhardtii, than to those of vascular plant species, but does not have the extra-long C-terminal sequence found in C. reinhardtii. When CO(2 )concentration was decreased from 3 to 0.04% (air level) in light, the levels of CkGBSS mRNA, CkGBSS protein, and GBSS activity increased. Under this condition, pyrenoid and pyrenoid starch developed, and the relative amount of amylose in starch increased. These observations suggest that low CO(2) level up-regulates GBSS biosynthesis at the transcriptional level.

Indexed as

Gene Expression Regulation, EnzymologicAlgal ProteinsAmino Acid SequenceCarbon DioxideChlorellaCloning, MolecularDNA, ComplementaryMolecular Sequence DataPhylogenyRNA, MessengerSequence AlignmentStarch SynthaseAlgal ProteinsCarbon DioxideDNA, ComplementaryRNA, MessengerStarch Synthase

Identifiers

PMID16482432
OpenAlexW2116691988

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.