ArticlePharmaceutical research2006
Molar-mass characterization of cationic polymers for gene delivery by aqueous size-exclusion chromatography.
Article in Pharmaceutical research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Mannosylated Polycations Target CD206Biomacromolecules · 2022Article
- Polymer Coated Oncolytic Adenovirus to Selectively Target Hepatocellular Carcinoma Cells.Pharmaceutics · 2021Article
- RGD-decorated cholesterol stabilized polyplexes for targeted siRNA delivery to glioblastoma cells.Drug delivery and translational research · 2019Article
- mRNA Polyplexes with Post-Conjugated GALA Peptides Efficiently Target, Transfect, and Activate Antigen Presenting Cells.Bioconjugate chemistry · 2019Article
- Stimuli-Responsive Cationic Hydrogels in Drug Delivery Applications.Gels (Basel, Switzerland) · 2018Review
- A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles.Nanoscale research letters · 2013Article
- Melittin-grafted HPMA-oligolysine based copolymers for gene delivery.Biomaterials · 2013Article
- Synthesis of statistical copolymers containing multiple functional peptides for nucleic Acid delivery.Biomacromolecules · 2010Article
- Synthesis and characterization of biodegradable HPMA-oligolysine copolymers for improved gene delivery.Bioconjugate chemistry · 2010Article
- Biodegradable poly(2-dimethylamino ethylamino)phosphazene for in vivo gene delivery to tumor cells. Effect of polymer molecular weight.Pharmaceutical research · 2007Article
Corrections and comments
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Authors and funding
7 authors.
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Abstract
purposeThis study was performed to develop a reliable aqueous size-exclusion chromatography (SEC) method to obtain the absolute molar masses and distributions of various cationic polymers used in gene delivery.
methodsWater-soluble cationic [2-(dimethylamino) ethyl methacrylate] polymers (PDEs) with different molar masses and low polydispersities were synthesized by living polymerization and these were used to optimize the SEC conditions. Online coupled multiangle light scattering (MALS) detection was applied to obtain the absolute molar masses. Narrow fractions of high molar mass were obtained by semipreparative SEC.
resultsIt was found that 0.3 M NaAc (pH 4.4) is a suitable eluent in combination with Shodex OHpak SB columns for SEC analysis of PDEs and other cationic polymers, such as poly(L-lysine) and poly(ethylene imine). The absolute molar masses of different PDEs were determined directly using SEC-MALS. A calibration curve was established using narrow PDEs.
conclusionsA reliable routine method for molar-mass characterization of cationic polymers was established. Because standards of known molar masses with narrow distributions are not commercially available for most polymers used in pharmaceutics and biotechnology, the procedure described in this work can also be applied for molar-mass characterization of other water-soluble polymers.
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