ArticleProceedings of the National Academy of Sciences of the United States of America1991
Mutagenesis of the myogenin basic region identifies an ancient protein motif critical for activation of myogenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
66 citing papers in PubMed, 158 citations in OpenAlex.
- Optimisation of cell fate determination for cultivated muscle differentiation.Communications biology · 2024Article
- Functional Characterization of an In-Frame Deletion in the Basic Domain of the Retinal Transcription Factor ATOH7.International journal of molecular sciences · 2022Article
- Mechanisms of Binding Specificity among bHLH Transcription Factors.International journal of molecular sciences · 2021Review
- Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts.Journal of animal science and biotechnology · 2021Article
- The Ciona myogenic regulatory factor functions as a typical MRF but possesses a novel N-terminus that is essential for activity.Developmental biology · 2019Article
- Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors.Development (Cambridge, England) · 2017Article
- Functional studies of the Ciona intestinalis myogenic regulatory factor reveal conserved features of chordate myogenesis.Developmental biology · 2013Article
- Chromatin immunoprecipitation assay for tissue-specific genes using early-stage mouse embryos.Journal of visualized experiments : JoVE · 2011Article
- MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.The Journal of biological chemistry · 2011Article
- Quadruplex structures of muscle gene promoter sequences enhance in vivo MyoD-dependent gene expression.Nucleic acids research · 2010Article
- Structure and functions of powerful transactivators: VP16, MyoD and FoxA.The International journal of developmental biology · 2010Review
- DNA binding-dependent and -independent functions of the Hand2 transcription factor during mouse embryogenesis.Development (Cambridge, England) · 2009Article
- Differential binding of quadruplex structures of muscle-specific genes regulatory sequences by MyoD, MRF4 and myogenin.Nucleic acids research · 2008Article
- Determinants of myogenic specificity within MyoD are required for noncanonical E box binding.Molecular and cellular biology · 2007Article
- MyoD uses overlapping but distinct elements to bind E-box and tetraplex structures of regulatory sequences of muscle-specific genes.Nucleic acids research · 2007Article
- The basic domain of ATH5 mediates neuron-specific promoter activity during retina development.Molecular and cellular biology · 2005Article
- The DNA binding activity of TAL-1 is not required to induce leukemia/lymphoma in mice.Oncogene · 2001Article
- Establishment of distinct MyoD, E2A, and twist DNA binding specificities by different basic region-DNA conformations.Molecular and cellular biology · 2000Article
- Activated notch inhibits myogenic activity of the MADS-Box transcription factor myocyte enhancer factor 2C.Molecular and cellular biology · 1999Article
- MyoR: a muscle-restricted basic helix-loop-helix transcription factor that antagonizes the actions of MyoD.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Myogenin is a muscle-specific nuclear factor that acts as a genetic switch to activate myogenesis. Myogenin, MyoD, and a growing number of proteins implicated in transcriptional control share sequence homology within a basic region and an adjacent helix-loop-helix motif. Here we identify by site-directed mutagenesis a 12-amino acid subdomain of the myogenin basic region essential for binding of DNA and activation of myogenesis. The basic region of the widely expressed helix-loop-helix protein E12 is conserved at 8 of these 12 residues and can mediate DNA binding when placed in myogenin, but it cannot activate myogenesis. Replacement of each of the four nonconserved residues of the myogenin basic region with the corresponding residues of E12 reveals two adjacent amino acids (Ala86-Thr) that can impart muscle specificity to the basic region. These residues are specific to, and conserved in, the basic regions of all known myogenic helix-loop-helix proteins from Drosophila to man, suggesting that they constitute part of an ancient protein motif required for activation of the myogenic program.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.