ArticleProceedings of the National Academy of Sciences of the United States of America2006
A complete ferredoxin/thioredoxin system regulates fundamental processes in amyloplasts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 176 citations in OpenAlex.
- Allelic variation in the thioredoxin TRX-M4 influences carbon partitioning to control soluble sugar content in apple fruit.Plant communications · 2026Article
- Plastidial phosphoglucose isomerase undergoes thioredoxin-mediated redox modification without altering catalytic activity.The Biochemical journal · 2025Article
- Old player, new roles: defining the role of the plastidial phosphorylase.The New phytologist · 2025Review
- Filamentous Temperature-Sensitive Z Protein J175 Regulates Maize Chloroplasts' and Amyloplasts' Division and Development.Plants (Basel, Switzerland) · 2025Article
- Spatiotemporal transcriptomics reveals key gene regulation for grain yield and quality in wheat.Genome biology · 2025Article
- Catalytic Activity of the Archetype from Group 4 of the FTR-like Ferredoxin:Thioredoxin Reductase Family Is Regulated by UniqueBiochemistry · 2024Article
- Current Insights into the Redox Regulation Network in Plant Chloroplasts.Plant & cell physiology · 2023Review
- NewAntioxidants (Basel, Switzerland) · 2022Article
- Molecular Functions and Pathways of Plastidial Starch Phosphorylase (PHO1) in Starch Metabolism: Current and Future Perspectives.International journal of molecular sciences · 2021Review
- The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation.Antioxidants (Basel, Switzerland) · 2021Article
- white panicle2 encoding thioredoxin z, regulates plastid RNA editing by interacting with multiple organellar RNA editing factors in rice.The New phytologist · 2021Article
- Biochemical insight into redox regulation of plastidial 3-phosphoglycerate dehydrogenase fromThe Journal of biological chemistry · 2020Article
- Tweaking Photosynthesis: FNR-TROL Interaction as Potential Target for Crop Fortification.Frontiers in plant science · 2020Article
- Proteomic Analyses of ThioredoxinsAntioxidants (Basel, Switzerland) · 2019Article
- Plant Immune Responses to Parasitic Nematodes.Frontiers in plant science · 2019Review
- Quantitative analysis of the grain amyloplast proteome reveals differences in metabolism between two wheat cultivars at two stages of grain development.BMC genomics · 2018Article
- Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms.International journal of molecular sciences · 2018Article
- Starch Trek: The Search for Yield.Frontiers in plant science · 2018Review
- Constitutive expression of a plant ferredoxin-like protein (pflp) enhances capacity of photosynthetic carbon assimilation in rice (Oryza sativa).Transgenic research · 2017Article
- Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A growing number of processes throughout biology are regulated by redox via thiol-disulfide exchange. This mechanism is particularly widespread in plants, where almost 200 proteins have been linked to thioredoxin (Trx), a widely distributed small regulatory disulfide protein. The current study extends regulation by Trx to amyloplasts, organelles prevalent in heterotrophic plant tissues that, among other biosynthetic activities, catalyze the synthesis and storage of copious amounts of starch. Using proteomics and immunological methods, we identified the components of the ferredoxin/Trx system (ferredoxin, ferredoxin-Trx reductase, and Trx), originally described for chloroplasts, in amyloplasts isolated from wheat starchy endosperm. Ferredoxin is reduced not by light, as in chloroplasts, but by metabolically generated NADPH via ferredoxin-NADP reductase. However, once reduced, ferredoxin appears to act as established for chloroplasts, i.e., via ferredoxin-Trx reductase and a Trx (m-type). A proteomics approach in combination with affinity chromatography and a fluorescent thiol probe led to the identification of 42 potential Trx target proteins, 13 not previously recognized, including a major membrane transporter (Brittle-1 or ADP-glucose transporter). The proteins function in a range of processes in addition to starch metabolism: biosynthesis of lipids, amino acids, and nucleotides; protein folding; and several miscellaneous reactions. The results suggest a mechanism whereby light is initially recognized as a thiol signal in chloroplasts, then as a sugar during transit to the sink, where it is converted again to a thiol signal. In this way, amyloplast reactions in the grain can be coordinated with photosynthesis taking place in leaves.
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