ArticleMolecular biology of the cell2006
The fission yeast transforming acidic coiled coil-related protein Mia1p/Alp7p is required for formation and maintenance of persistent microtubule-organizing centers at the nuclear envelope.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Human Cytomegalovirus Exploits TACC3 To Control Microtubule Dynamics and Late Stages of Infection.Journal of virology · 2021Article
- TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells.Cell reports · 2020Article
- Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope.Journal of molecular cell biology · 2019Article
- The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.Current biology : CB · 2018Article
- Article
- Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism.Scientific reports · 2016Article
- A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit.The Journal of cell biology · 2015Article
- The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.Molecular biology of the cell · 2013Article
- Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast.EMBO reports · 2009Article
- Nucleocytoplasmic shuttling of the TACC protein Mia1p/Alp7p is required for remodeling of microtubule arrays during the cell cycle.PloS one · 2009Article
- Force- and kinesin-8-dependent effects in the spatial regulation of fission yeast microtubule dynamics.Molecular systems biology · 2009Article
- Xenopus TACC3/maskin is not required for microtubule stability but is required for anchoring microtubules at the centrosome.Molecular biology of the cell · 2008Article
- The spindle pole bodies facilitate nuclear envelope division during closed mitosis in fission yeast.PLoS biology · 2007Article
- Microtubule-associated proteins in higher plants.Journal of plant research · 2007Review
- Cytoplasmic microtubule organization in fission yeast.Yeast (Chichester, England) · 2006Review
- Gamma-tubulin is essential for acentrosomal microtubule nucleation and coordination of late mitotic events in Arabidopsis.The Plant cell · 2006Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Microtubule-organizing centers (MTOCs) concentrate microtubule nucleation, attachment and bundling factors and thus restrict formation of microtubule arrays in spatial and temporal manner. How MTOCs occur remains an exciting question in cell biology. Here, we show that the transforming acidic coiled coil-related protein Mia1p/Alp7p functions in emergence of large MTOCs in interphase fission yeast cells. We found that Mia1p was a microtubule-binding protein that preferentially localized to the minus ends of microtubules and was associated with the sites of microtubule attachment to the nuclear envelope. Cells lacking Mia1p exhibited less microtubule bundles. Microtubules could be nucleated and bundled but were frequently released from the nucleation sites in mia1delta cells. Mia1p was required for stability of microtubule bundles and persistent use of nucleation sites both in interphase and postanaphase array dynamics. The gamma-tubulin-rich material was not organized in large perinuclear or microtubule-associated structures in mia1delta cells. Interestingly, absence of microtubules in dividing wild-type cells prevented appearance of large gamma-tubulin-rich MTOC structures in daughters. When microtubule polymerization was allowed, MTOCs were efficiently assembled de novo. We propose a model where MTOC emergence is a self-organizing process requiring the continuous association of microtubules with nucleation sites.
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Registered trials
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