ArticleMolecular biology of the cell2006
The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Synaptotagmin-like 5 is a potential biomarker and correlates with immune infiltrates in thyroid carcinoma.Journal of molecular histology · 2025Article
- Immunohistochemical field parcellation of the human hippocampus along its antero-posterior axis.Brain structure & function · 2024Article
- Overlapping Machinery in Lysosome-Related Organelle Trafficking: A Lesson from Rare Multisystem Disorders.Cells · 2022Review
- Identification of genetic mechanisms for tissue-specific genetic effects based on CRISPR screens.Genetics · 2022Article
- Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- SYTL4 downregulates microtubule stability and confers paclitaxel resistance in triple-negative breast cancer.Theranostics · 2020Article
- Small Interfering RNA Screening for the Small GTPase Rab Proteins Identifies Rab5B as a Major Regulator of Hepatitis B Virus Production.Journal of virology · 2019Article
- A Rab3a-dependent complex essential for lysosome positioning and plasma membrane repair.The Journal of cell biology · 2016Article
- Granuphilin exclusively mediates functional granule docking to the plasma membrane.Scientific reports · 2016Article
- Resident CAPS on dense-core vesicles docks and primes vesicles for fusion.Molecular biology of the cell · 2016Article
- Rab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate.The Journal of cell biology · 2015Article
- Distinct actions of Rab3 and Rab27 GTPases on late stages of exocytosis of insulin.Traffic (Copenhagen, Denmark) · 2014Article
- The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids.Chemistry and physics of lipids · 2014Article
- The secretion of von Willebrand factor from endothelial cells; an increasingly complicated story.Journal of thrombosis and haemostasis : JTH · 2013Review
- The Rab27a effector exophilin7 promotes fusion of secretory granules that have not been docked to the plasma membrane.Molecular biology of the cell · 2013Article
- Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells.Nature cell biology · 2012Article
- Structure-function study of mammalian Munc18-1 and C. elegans UNC-18 implicates domain 3b in the regulation of exocytosis.PloS one · 2011Article
- Article
- Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein.Molecular biology of the cell · 2010Article
- CAPS drives trans-SNARE complex formation and membrane fusion through syntaxin interactions.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptotagmin-like protein 4-a (Slp4-a)/granuphilin-a is specifically localized on dense-core vesicles in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. However, the precise molecular mechanism of its inhibitory effect on exocytosis has never been elucidated and is still a matter of controversy. Here we show by deletion and chimeric analyses that the linker domain of Slp4-a interacts with the Munc18-1.syntaxin-1a complex by directly binding to Munc18-1 and that this interaction promotes docking of dense-core vesicles to the plasma membrane in PC12 cells. Despite increasing the number of plasma membrane docked vesicles, expression of Slp4-a strongly inhibited high-KCl-induced dense-core vesicle exocytosis. The inhibitory effect by Slp4-a is absolutely dependent on the linker domain of Slp4-a, because substitution of the linker domain of Slp4-a by that of Slp5 (the closest isoform of Slp4-a that cannot bind the Munc18-1.syntaxin-1a complex) completely abrogated the inhibitory effect. Our findings reveal a novel docking machinery for dense-core vesicle exocytosis: Slp4-a simultaneously interacts with Rab27A and Munc18-1 on the dense-core vesicle and with syntaxin-1a in the plasma membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.