ArticleNucleic acids research2006
Changes in DNA bending and flexing due to tethered cations detected by fluorescence resonance energy transfer.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Mechanical Flexibility of DNA: A Quintessential Tool for DNA Nanotechnology.Sensors (Basel, Switzerland) · 2020Review
- DNA curvature and flexibility in vitro and in vivo.Quarterly reviews of biophysics · 2010Review
- DNA on a tube: electrostatic contribution to stiffness.The journal of physical chemistry. B · 2008Article
- Monovalent cation binding by curved DNA molecules containing variable numbers of a-tracts.Biophysical journal · 2008Article
- Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method.Biochemistry · 2007Article
- A review of the role of the sequence-dependent electrostatic landscape in DNA alkylation patterns.Chemical research in toxicology · 2006Review
- Spontaneous sharp bending of DNA: role of melting bubbles.Nucleic acids research · 2006Article
- DNA bending by bHLH charge variants.Nucleic acids research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Local DNA deformation arises from an interplay among sequence-related base stacking, intrastrand phosphate repulsion, and counterion and water distribution, which is further complicated by the approach and binding of a protein. The role of electrostatics in this complex chemistry was investigated using tethered cationic groups that mimic proximate side chains. A DNA duplex was modified with one or two centrally located deoxyuracils substituted at the 5-position with either a flexible 3-aminopropyl group or a rigid 3-aminopropyn-1-yl group. End-to-end helical distances and duplex flexibility were obtained from measurements of the time-resolved Förster resonance energy transfer between 5'- and 3'-linked dye pairs. A novel analysis utilized the first and second moments of the G(t) function, which encompasses only the energy transfer process. Duplex flexibility is altered by the presence of even a single positive charge. In contrast, the mean 5'-3' distance is significantly altered by the introduction of two adjacently tethered cations into the double helix but not by a single cation: two adjacent aminopropyl groups decrease the 5'-3' distance while neighboring aminopropynyl groups lengthen the helix.
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Registered trials
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