ReviewMolecular aspects of medicine
Detection and monitoring of virus infections by real-time PCR.
Review in Molecular aspects of medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
100 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Incidence of Cytomegalovirus (CMV) Infection in After Kidney Transplant Patients: A Systematic Review and Meta-Analysis.Reviews in medical virology · 2026Pooled it
- Accuracy of real-time PCR assays for human papillomavirus using urine samples: a systematic review and meta-analysis.Journal of clinical microbiology · 2025Pooled it
- Surface-Enhanced Raman Spectroscopy for Viral Diagnostics: Principles, Strategies, Clinical Challenges, and Future Directions.Chemical reviews · 2026Review
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
- Detection Strategies for Begomoviruses: A Focus Review of Methods.Molecular biotechnology · 2026Review
- Morphology-guided deep learning for nanoparticle agglomeration diagnostic assays.Scientific reports · 2026Article
- Long-Term Stable Biosensing Using Multiscale Biostructure-Preserving Metal Thin Films.Biosensors · 2026Article
- Viral Sentry AI-Automated zoonotic surveillance and drug repurposing agent.Biology methods & protocols · 2026Article
- Quality control strategy for differentiating induced pluripotent stem cells into pancreatic β-cells before transplantation.Regenerative medicine · 2026Review
- Article
- First Isolation of Bovine Coronavirus From Yanbian, China, and Analytical Validation of a SYBR Green I RT-qPCR Panel for Calf Diarrhea Viruses.Transboundary and emerging diseases · 2026Article
- Development of a TaqMan based real-time PCR assay for the detection of bovine ephemeral fever virus.Molecular biology reports · 2025Article
- High Concordance Between SYBR Green and TaqMan PCR for SARS-CoV-2 Detection in Nasopharyngeal and Saliva Samples.Viruses · 2025Article
- Development and Evaluation of a Nanoparticle-Based Immunoassay for Rotavirus Detection: A Suitable Alternative to ELISA and PCR in Low-Income Setting.Methods and protocols · 2025Article
- Optical Method for the Detection of Viral RNA Using an Optical Fiber Sensor.Journal of biophotonics · 2025Article
- Development of an RT-RPA assay for La Crosse virus detection provides insights into age-dependent neuroinvasion in mice.Virology journal · 2025Article
- Real-Time Polymerase Chain Reaction Systems for Detection and Differentiation of Unclassified Viruses of theMethods and protocols · 2025Article
- Review
- Canine distemper outbreak and laryngeal paralysis in captive tigers (Panthera tigris).BMC veterinary research · 2025Article
- Geminivirus diseases of legumes in India: current status and approaches for management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The employment of polymerase chain reaction (PCR) techniques for virus detection and quantification offers the advantages of high sensitivity and reproducibility, combined with an extremely broad dynamic range. A number of qualitative and quantitative PCR virus assays have been described, but commercial PCR kits are available for quantitative analysis of a limited number of clinically important viruses only. In addition to permitting the assessment of viral load at a given time point, quantitative PCR tests offer the possibility of determining the dynamics of virus proliferation, monitoring of the response to treatment and, in viruses displaying persistence in defined cell types, distinction between latent and active infection. Moreover, from a technical point of view, the employment of sequential quantitative PCR assays in virus monitoring helps identifying false positive results caused by inadvertent contamination of samples with traces of viral nucleic acids or PCR products. In this review, we provide a survey of the current state-of-the-art in the application of the real-time PCR technology to virus analysis. Advantages and limitations of the RQ-PCR methodology, and quality control issues related to standardization and validation of diagnostic assays are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.