ArticleBMC genomics2006
Pooled DNA genotyping on Affymetrix SNP genotyping arrays.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed.
- FREQ-Seq2: a method for precise high-throughput combinatorial quantification of allele frequencies.G3 (Bethesda, Md.) · 2023Article
- Deciphering the Genetic Diversity of Landraces With High-Throughput SNP Genotyping of DNA Bulks: Methodology and Application to the Maize 50k Array.Frontiers in plant science · 2020Article
- Identification of novel alleles associated with insulin resistance in childhood obesity using pooled-DNA genome-wide association study approach.International journal of obesity (2005) · 2018Article
- A Novel Relationship for Schizophrenia, Bipolar, and Major Depressive Disorder. Part 8: a Hint from Chromosome 8 High Density Association Screen.Molecular neurobiology · 2017Article
- A genome-wide association study of essential hypertension in an Australian population using a DNA pooling approach.Molecular genetics and genomics : MGG · 2017Article
- A novel relationship for schizophrenia, bipolar and major depressive disorder Part 5: a hint from chromosome 5 high density association screen.American journal of translational research · 2017Article
- Article
- Whole-miRNome profiling identifies prognostic serum miRNAs in esophageal adenocarcinoma: the influence of Helicobacter pylori infection status.Carcinogenesis · 2015Article
- A novel candidate region for genetic adaptation to high altitude in Andean populations.PloS one · 2015Article
- Disentangling pooled triad genotypes for association studies.Annals of human genetics · 2014Article
- Fish scales and SNP chips: SNP genotyping and allele frequency estimation in individual and pooled DNA from historical samples of Atlantic salmon (Salmo salar).BMC genomics · 2013Article
- Assignment of SNP allelic configuration in polyploids using competitive allele-specific PCR: application to citrus triploid progeny.Annals of botany · 2013Article
- Genome wide allele frequency fingerprints (GWAFFs) of populations via genotyping by sequencing.PloS one · 2013Article
- Evaluation of quality of DNA extracted from buccal swabs for microarray based genotyping.Indian journal of clinical biochemistry : IJCB · 2012Article
- FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations.PloS one · 2012Article
- A three-stage genome-wide association study of general cognitive ability: hunting the small effects.Behavior genetics · 2010Article
- Utility of the pooling approach as applied to whole genome association scans with high-density Affymetrix microarrays.BMC research notes · 2010Article
- Genome-wide association study identifies genes that may contribute to risk for developing heroin addiction.Psychiatric genetics · 2010Article
- Article
- A genome-wide association study identifies multiple loci associated with mathematics ability and disability.Genes, brain, and behavior · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundGenotyping technology has advanced such that genome-wide association studies of complex diseases based upon dense marker maps are now technically feasible. However, the cost of such projects remains high. Pooled DNA genotyping offers the possibility of applying the same technologies at a fraction of the cost, and there is some evidence that certain ultra-high throughput platforms also perform with an acceptable accuracy. However, thus far, this conclusion is based upon published data concerning only a small number of SNPs.
resultsIn the current study we prepared DNA pools from the parents and from the offspring of 30 parent-child trios that have been extensively genotyped by the HapMap project. We analysed the two pools with Affymetrix 10 K Xba 142 2.0 Arrays. The availability of the HapMap data allowed us to validate the performance of 6843 SNPs for which we had both complete individual and pooled genotyping data. Pooled analyses averaged over 5-6 microarrays resulted in highly reproducible results. Moreover, the accuracy of estimating differences in allele frequency between pools using this ultra-high throughput system was comparable with previous reports of pooling based upon lower throughput platforms, with an average error for the predicted allelic frequencies differences between the two pools of 1.37% and with 95% of SNPs showing an error of < 3.2%.
conclusionGenotyping thousands of SNPs with DNA pooling using Affymetrix microarrays produces highly accurate results and can be used for genome-wide association studies.
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