ArticleMolecular and cellular biology2006
A CTCF-dependent silencer located in the differentially methylated area may regulate expression of a housekeeping gene overlapping a tissue-specific gene domain.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Uncovering the whole genome silencers of human cells via Ss-STARR-seq.Nature communications · 2025Article
- DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells.EMBO molecular medicine · 2024Article
- Identification of non-coding silencer elements and their regulation of gene expression.Nature reviews. Molecular cell biology · 2023Review
- ZNF555 protein binds to transcriptional activator site of 4qA allele and ANT1: potential implication in Facioscapulohumeral dystrophy.Nucleic acids research · 2015Article
- Article
- The clustering of CpG islands may constitute an important determinant of the 3D organization of interphase chromosomes.Epigenetics · 2014Article
- A long non-coding RNA promotes full activation of adult gene expression in the chicken α-globin domain.Epigenetics · 2014Article
- CTCF demarcates chicken embryonic α-globin gene autonomous silencing and contributes to adult stage-specific gene expression.Epigenetics · 2013Article
- QDMR: a quantitative method for identification of differentially methylated regions by entropy.Nucleic acids research · 2011Article
- An insulator embedded in the chicken α-globin locus regulates chromatin domain configuration and differential gene expression.Nucleic acids research · 2011Article
- Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedure.Nucleic acids research · 2010Article
- TMEM8 - a non-globin gene entrapped in the globin web.Nucleic acids research · 2009Article
- A histone map of human chromosome 20q13.12.PloS one · 2009Article
- Spatial configuration of the chicken alpha-globin gene domain: immature and active chromatin hubs.Nucleic acids research · 2008Article
- Epigenetic boundaries of tumour suppressor gene promoters: the CTCF connection and its role in carcinogenesis.Journal of cellular and molecular medicineReview
- Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tissue-specific chicken alpha-globin gene domain represents one of the paradigms, in terms of its constitutively open chromatin conformation and the location of several regulatory elements within the neighboring housekeeping gene. Here, we show that an 0.2-kb DNA fragment located approximately 4 kb upstream to the chicken alpha-globin gene cluster contains a binding site for the multifunctional protein factor CTCF and possesses silencer activity which depends on CTCF binding, as demonstrated by site-directed mutagenesis of the CTCF recognition sequence. CTCF was found to be associated with this recognition site in erythroid cells but not in lymphoid cells where the site is methylated. A functional promoter directing the transcription of the apparently housekeeping ggPRX gene was found 120 bp from the CTCF-dependent silencer. The data are discussed in terms of the hypothesis that the CTCF-dependent silencer stabilizes the level of ggPRX gene transcription in erythroid cells where the promoter of this gene may be influenced by positive cis-regulatory signals activating alpha-globin gene transcription.
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