ArticleNucleic acids research2006
Affinity selection of DNA-binding protein complexes using mRNA display.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
- C-Terminal Tag Location Hampers in Vitro Profiling of OGT Peptide Substrates by mRNA Display.Chembiochem : a European journal of chemical biology · 2021Article
- The science of puromycin: From studies of ribosome function to applications in biotechnology.Computational and structural biotechnology journal · 2020Review
- Anti-inflammatory effects of adenosine N1-oxide.Journal of inflammation (London, England) · 2015Article
- In vitro selection of proteins with desired characteristics using mRNA-display.Methods (San Diego, Calif.) · 2013Article
- An anilinoquinazoline derivative inhibits tumor growth through interaction with hCAP-G2, a subunit of condensin II.PloS one · 2012Article
- mRNA display-based selections using synthetic peptide and natural protein libraries.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Selection of proteins with desired properties from natural proteome libraries using mRNA display.Nature protocols · 2011Article
- Advantages of mRNA display selections over other selection techniques for investigation of protein-protein interactions.Expert review of proteomics · 2011Review
- Rapid antibody selection by mRNA display on a microfluidic chip.Nucleic acids research · 2009Article
- Use of cDNA tiling arrays for identifying protein interactions selected by in vitro display technologies.PloS one · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive analysis of DNA-protein interactions is important for mapping transcriptional regulatory networks on a genome-wide level. Here we present a new application of mRNA display for in vitro selection of DNA-binding protein heterodimeric complexes. Under improved selection conditions using a TPA-responsive element (TRE) as a bait DNA, known interactors c-fos and c-jun were simultaneously enriched about 100-fold from a model library (a 1:1:20 000 mixture of c-fos, c-jun and gst genes) after one round of selection. Furthermore, almost all kinds of the AP-1 family genes including c-jun, c-fos, junD, junB, atf2 and b-atf were successfully selected from an mRNA display library constructed from a mouse brain poly A(+) RNA after six rounds of selection. These results indicate that the mRNA display selection system can identify a variety of DNA-binding protein complexes in a single experiment. Since almost all transcription factors form heterooligomeric complexes to bind with their target DNA, this method should be most useful to search for DNA-binding transcription factor complexes.
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