ArticleNucleic acids research2006
High-throughput trapping of secretory pathway genes in mouse embryonic stem cells.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Mutant non-coding RNA resource in mouse embryonic stem cells.Disease models & mechanisms · 2021Article
- PiggyBac transposon-based polyadenylation-signal trap for genome-wide mutagenesis in mice.Scientific reports · 2016Article
- Conditionals by inversion provide a universal method for the generation of conditional alleles.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- ERO1-beta, a pancreas-specific disulfide oxidase, promotes insulin biogenesis and glucose homeostasis.The Journal of cell biology · 2010Article
- Gene trap mutagenesis in the mouse.Methods in enzymology · 2010Article
- Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells.PloS one · 2007Article
- A novel view of the transcriptome revealed from gene trapping in mouse embryonic stem cells.Genome research · 2007Article
- Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The carnegie protein trap library: a versatile tool for Drosophila developmental studies.Genetics · 2007Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput gene trapping is a random approach for inducing insertional mutations across the mouse genome. This approach uses gene trap vectors that simultaneously inactivate and report the expression of the trapped gene at the insertion site, and provide a DNA tag for the rapid identification of the disrupted gene. Gene trapping has been used by both public and private institutions to produce libraries of embryonic stem (ES) cells harboring mutations in single genes. Presently, approximately 66% of the protein coding genes in the mouse genome have been disrupted by gene trap insertions. Among these, however, genes encoding signal peptides or transmembrane domains (secretory genes) are underrepresented because they are not susceptible to conventional trapping methods. Here, we describe a high-throughput gene trapping strategy that effectively targets secretory genes. We used this strategy to assemble a library of ES cells harboring mutations in 716 unique secretory genes, of which 61% were not trapped by conventional trapping, indicating that the two strategies are complementary. The trapped ES cell lines, which can be ordered from the International Gene Trap Consortium (http://www.genetrap.org), are freely available to the scientific community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.