ArticleProceedings of the National Academy of Sciences of the United States of America2006
DNA conformation on surfaces measured by fluorescence self-interference.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Integrated Spectral Sensitivity as Physics-Based Figure of Merit for Spectral Transducers in Optical Sensing.Sensors (Basel, Switzerland) · 2025Article
- Axial localization and tracking of self-interference nanoparticles by lateral point spread functions.Nature communications · 2021Article
- A force sensor that converts fluorescence signal into force measurement utilizing short looped DNA.Biosensors & bioelectronics · 2018Article
- BIOPHYSICAL PROPERTIES OF NUCLEIC ACIDS AT SURFACES RELEVANT TO MICROARRAY PERFORMANCE.Biomaterials science · 2014Article
- 4Pi fluorescence detection and 3D particle localization with a single objective.Optics express · 2013Article
- Efficient self-assembly of DNA-functionalized fluorophores and gold nanoparticles with DNA functionalized silicon surfaces: the effect of oligomer spacers.Nucleic acids research · 2013Article
- Entropy-driven collective interactions in DNA brushes on a biochip.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A new microarray substrate for ultra-sensitive genotyping of KRAS and BRAF gene variants in colorectal cancer.PloS one · 2013Article
- Free energy considerations for nucleic acids with dangling ends near a surface: a coarse grained approach.Journal of physics. Condensed matter : an Institute of Physics journal · 2011Article
- A model for Structure and Thermodynamics of ssDNA and dsDNA Near a Surface: a Coarse Grained Approach.Computer physics communications · 2010Article
- Platform for in situ real-time measurement of protein-induced conformational changes of DNA.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Quantification of DNA and protein adsorption by optical phase shift.Biosensors & bioelectronics · 2009Article
- Direct observation of conformation of a polymeric coating with implications in microarray applications.Analytical chemistry · 2009Article
- Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface.Nucleic acids research · 2008Article
- Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- DNA surface hybridization regimes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Quantitative determination of size and shape of surface-bound DNA using an acoustic wave sensor.Biophysical journal · 2008Article
- Fluorescence interferometry: principles and applications in biology.Annals of the New York Academy of Sciences · 2008Review
- Switchable DNA interfaces for the highly sensitive detection of label-free DNA targets.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The conformation of DNA molecules tethered to the surface of a microarray may significantly affect the efficiency of hybridization. Although a number of methods have been applied to determine the structure of the DNA layer, they are not very sensitive to variations in the shape of DNA molecules. Here we describe the application of an interferometric technique called spectral self-interference fluorescence microscopy to the precise measurement of the average location of a fluorescent label in a DNA layer relative to the surface and thus determine specific information on the conformation of the surface-bound DNA molecules. Using spectral self-interference fluorescence microscopy, we have estimated the shape of coiled single-stranded DNA, the average tilt of double-stranded DNA of different lengths, and the amount of hybridization. The data provide important proofs of concept for the capabilities of novel optical surface analytical methods of the molecular disposition of DNA on surfaces. The determination of DNA conformations on surfaces and hybridization behavior provide information required to move DNA interfacial applications forward and thus impact emerging clinical and biotechnological fields.
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