ArticleTransgenic research2006
Homologous recombination in zebrafish ES cells.
Article in Transgenic research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 65 citations in OpenAlex.
- Stage-Specific Effects of Ionizing Radiation during Early Development.International journal of molecular sciences · 2020Review
- Gene replacement by zinc finger nucleases in medaka embryos.Marine biotechnology (New York, N.Y.) · 2014Article
- Sgs1 and Exo1 suppress targeted chromosome duplication during ends-in and ends-out gene targeting.DNA repair · 2014Article
- Zebrafish models of human liver development and disease.Comprehensive Physiology · 2013Review
- Article
- Nanos3 gene targeting in medaka ES cells.International journal of biological sciences · 2013Article
- Study of pluripotency markers in zebrafish embryos and transient embryonic stem cell cultures.Zebrafish · 2011Article
- Investigating the genetics of visual processing, function and behaviour in zebrafish.Neurogenetics · 2011Review
- Fish stem cell cultures.International journal of biological sciences · 2011Review
- Use of phage φC31 integrase as a tool for zebrafish genome manipulation.Methods in cell biology · 2011Article
- Somatic cell nuclear transfer in zebrafish.Nature methods · 2009Article
- Progress and prospects: techniques for site-directed mutagenesis in animal models.Gene therapy · 2009Review
- New technology for an old favorite: lentiviral transgenesis and RNAi in rats.Transgenic research · 2007Review
- Efficient transfection of primary zebrafish fibroblasts by nucleofection.Cytotechnology · 2006Article
- Generation of medaka gene knockout models by target-selected mutagenesis.Genome biology · 2006Article
- Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus).Marine biotechnology (New York, N.Y.)Article
- Site-directed gene integration in transgenic zebrafish mediated by cre recombinase using a combination of mutant lox sites.Marine biotechnology (New York, N.Y.)Article
- Fish ES cells and applications to biotechnology.Marine biotechnology (New York, N.Y.)Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Targeted insertion of a plasmid by homologous recombination was demonstrated in zebrafish ES cell cultures. Two selection strategies were used to isolate ES cell colonies that contained targeted plasmid insertions in either the no tail or myostatin I gene. One selection strategy involved the manual isolation of targeted cell colonies that were identified by the loss of fluorescent protein gene expression. A second strategy used the diphtheria toxin A-chain gene in a positive-negative selection approach. Homologous recombination was confirmed by PCR, sequence and Southern blot analysis and colonies isolated using both selection methods were expanded and maintained for multiple passages. The results demonstrate that zebrafish ES cells have potential for use in a cell-mediated gene targeting approach.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.