ReviewNucleic acids research2006
Options available for profiling small samples: a review of sample amplification technology when combined with microarray profiling.
Review in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Trial
- Trial
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- A Detailed Overview About the Single-Cell Analyses of Solid Tumors Focusing on Colorectal Cancer.Pathology oncology research : POR · 2022Review
- Retrospective analysis of cancer-specific gene expression panel for thyroid fine needle aspiration specimens.Journal of cancer research and clinical oncology · 2021Article
- Microfluidics for genome-wide studies involving next generation sequencing.Biomicrofluidics · 2017Review
- Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform.Journal of visualized experiments : JoVE · 2017Article
- Protein synthetic machinery and mRNA in regenerating tips of spinal cord axons in lamprey.The Journal of comparative neurology · 2016Article
- Article
- Transcriptional analysis of micro-dissected articular cartilage in post-traumatic murine osteoarthritis.Osteoarthritis and cartilage · 2015Article
- Single-cell RNA-seq: advances and future challenges.Nucleic acids research · 2014Review
- MicroRNA-769-3p down-regulates NDRG1 and enhances apoptosis in MCF-7 cells during reoxygenation.Scientific reports · 2014Article
- Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2014Article
- Adjustment method for microarray data generated using two-cycle RNA labeling protocol.BMC genomics · 2013Article
- Single-cell and regional gene expression analysis in Alzheimer's disease.Cellular and molecular neurobiology · 2012Review
- Article
- Systems biology of fungal infection.Frontiers in microbiology · 2012Article
- Article
- Article
- Extraction of bacterial RNA from soil: challenges and solutions.Microbes and environments · 2012Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The possibility of performing microarray analysis on limited material has been demonstrated in a number of publications. In this review we approach the technical aspects of mRNA amplification and several important implicit consequences, for both linear and exponential procedures. Amplification efficiencies clearly allow profiling of extremely small samples. The conservation of transcript abundance is the most important issue regarding the use of sample amplification in combination with microarray analysis, and this aspect has generally been found to be acceptable, although demonstrated to decrease in highly diluted samples. The fact that variability and discrepancies in microarray profiles increase with minute sample sizes has been clearly documented, but for many studies this does appear to have affected the biological conclusions. We suggest that this is due to the data analysis approach applied, and the consequence is the chance of presenting misleading results. We discuss the issue of amplification sensitivity limits in the light of reports on fidelity, published data from reviewed articles and data analysis approaches. These are important considerations to be reflected in the design of future studies and when evaluating biological conclusions from published microarray studies based on extremely low input RNA quantities.
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