ArticleRetrovirology2006
Intracellular assembly and budding of the Murine Leukemia Virus in infected cells.
Article in Retrovirology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Translation of unspliced retroviral genomic RNA in the host cell is regulated in both space and time.The Journal of cell biology · 2025Article
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- Unique Aggregation of Retroviral Particles Pseudotyped with the Delta Variant SARS-CoV-2 Spike Protein.Viruses · 2022Article
- The Delta variant SARS-CoV-2 spike protein uniquely promotes aggregation of pseudotyped viral particles.bioRxiv : the preprint server for biology · 2022Article
- Review
- Post-Translational Modifications of Retroviral HIV-1 Gag Precursors: An Overview of Their Biological Role.International journal of molecular sciences · 2021Review
- Selective Isolation of Retroviruses from Extracellular Vesicles by Intact Virion Immunoprecipitation.Bio-protocol · 2018Article
- Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry.Scientific reports · 2017Article
- ESCRT Requirements for Murine Leukemia Virus Release.Viruses · 2016Article
- Equine Infectious Anemia Virus Gag Assembly and Export Are Directed by Matrix Protein through trans-Golgi Networks and Cellular Vesicles.Journal of virology · 2016Article
- Electron microscopy: essentials for viral structure, morphogenesis and rapid diagnosis.Science China. Life sciences · 2013Review
- Multiple Gag domains contribute to selective recruitment of murine leukemia virus (MLV) Env to MLV virions.Journal of virology · 2013Article
- Article
- Implications of the nucleocapsid and the microenvironment in retroviral reverse transcription.Viruses · 2010Article
- Targeting of murine leukemia virus gag to the plasma membrane is mediated by PI(4,5)P2/PS and a polybasic region in the matrix.Journal of virology · 2010Article
- When is it time for reverse transcription to start and go?Retrovirology · 2009Review
- Intracellular HIV-1 Gag localization is impaired by mutations in the nucleocapsid zinc fingers.Retrovirology · 2007Article
- Plasma membrane is the site of productive HIV-1 particle assembly.PLoS biology · 2006Article
- Cell biology of retroviral RNA packaging.RNA biologyReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMurine Leukemia Virus (MLV) assembly has been long thought to occur exclusively at the plasma membrane. Current models of retroviral particle assembly describe the recruitment of the host vacuolar protein sorting machinery to the cell surface to induce the budding of new particles. Previous fluorescence microscopy study reported the vesicular traffic of the MLV components (Gag, Env and RNA). Here, electron microscopy (EM) associated with immunolabeling approaches were used to go deeply into the assembly of the "prototypic" MLV in chronically infected NIH3T3 cells.
resultsBeside the virus budding events seen at the cell surface of infected cells, we observed that intracellular budding events could also occur inside the intracellular vacuoles in which many VLPs accumulated. EM in situ hybridization and immunolabeling analyses confirmed that these latter were MLV particles. Similar intracellular particles were detected in cells expressing MLV Gag alone. Compartments containing the MLV particles were identified as late endosomes using Lamp1 endosomal/lysosomal marker and BSA-gold pulse-chase experiments. In addition, infectious activity was detected in lysates of infected cells.
conclusionAltogether, our results showed that assembly of MLV could occur in part in intracellular compartments of infected murine cells and participate in the production of infectious viruses. These observations suggested that MLV budding could present similarities with the particular intracellular budding of HIV in infected macrophages.
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