ArticleMolecular genetics and genomics : MGG2006
Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae.
Article in Molecular genetics and genomics : MGG, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
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- Engineering Industrial Strain ofAntibiotics (Basel, Switzerland) · 2026Article
- Recent Advances in Heterologous Protein Expression and Natural Product Synthesis byJournal of fungi (Basel, Switzerland) · 2025Review
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Synthetic biology meets Aspergillus: engineering strategies for next-generation organic acid production.World journal of microbiology & biotechnology · 2025Review
- Co-transformation of Aspergillus fumigatus: a simple and efficient strategy for gene editing without linking selectable markers.Access microbiology · 2025Article
- Advancements in lipid production research using the koji-moldFrontiers in fungal biology · 2024Review
- CRISPR/Cas9 improves targeted knock-in efficiency inBiotechnology notes (Amsterdam, Netherlands) · 2024Article
- Strategies for the Development of Industrial Fungal Producing Strains.Journal of fungi (Basel, Switzerland) · 2023Review
- Identification of sugar transporter genes and their roles in the pathogenicity ofFrontiers in plant science · 2023Article
- CRISPR/Cas9-Based Genome Editing and Its Application inJournal of fungi (Basel, Switzerland) · 2022Review
- Concomitant knockout of target and transporter genes in filamentous fungi by genome co-editing.MicrobiologyOpen · 2022Article
- A Homologous Recombination System to Generate Epitope-Tagged Target Genes inInternational journal of molecular sciences · 2022Article
- NIH4215: A mutation-prone thiamine auxotrophic clinicalFrontiers in fungal biology · 2022Article
- Major involvement of two laccase genes in conidial pigment biosynthesis in Aspergillus oryzae.Applied microbiology and biotechnology · 2022Article
- Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9.Applied and environmental microbiology · 2021Article
- Genome Editing Technology and Its Application Potentials in the Industrial Filamentous FungusJournal of fungi (Basel, Switzerland) · 2021Review
- Updates inJournal of fungi (Basel, Switzerland) · 2021Review
- Metabolic Engineering Techniques to Increase the Productivity of Primary and Secondary Metabolites Within Filamentous Fungi.Frontiers in fungal biology · 2021Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
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Abstract
In the koji molds Aspergillus sojae and Aspergillus oryzae, exogenous DNA is integrated in the genome, in most cases irrespective of the sequence homology, suggesting that DNA integration occurs predominantly through a nonhomologous end joining pathway where two ku genes, namely, ku70 and ku80, play a key role. To determine the effect of ku gene disruption on the gene targeting frequency, we constructed ku70-, ku80-, and ku70-ku80-disrupted strains of A. sojae and A. oryzae. The gene targeting frequency of the tannase gene in ku70 and ku80 strains of both Aspergillus species was markedly enhanced as compared with that of the parental strains. The gene targeting frequency of the aflR and ku80 genes was also enhanced in an A. sojae ku70 background. Therefore, the koji mold strains with ku-disrupted genes will be excellent tools as hosts for efficient gene targeting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.