ArticleThe Journal of physiology2006
Removal of gating in voltage-dependent ClC-2 chloride channel by point mutations affecting the pore and C-terminus CBS-2 domain.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Identification of a novelFrontiers in genetics · 2026Article
- Article
- CryoEM structures of the human CLC-2 voltage gated chloride channel reveal a ball and chain gating mechanism.bioRxiv : the preprint server for biology · 2023Article
- Cryo-EM structures of ClC-2 chloride channel reveal the blocking mechanism of its specific inhibitor AK-42.Nature communications · 2023Article
- Electro-steric opening of the CLC-2 chloride channel gate.Scientific reports · 2021Article
- Regulatory Conformational Coupling between CLC Anion Channel Membrane and Cytoplasmic Domains.Biophysical journal · 2016Article
- Dual regulation of the native ClC-K2 chloride channel in the distal nephron by voltage and pH.The Journal of general physiology · 2016Article
- GlialCAM, a CLC-2 Cl(-) channel subunit, activates the slow gate of CLC chloride channels.Biophysical journal · 2014Article
- CLCNKB mutations causing mild Bartter syndrome profoundly alter the pH and Ca2+ dependence of ClC-Kb channels.Pflugers Archiv : European journal of physiology · 2014Article
- Common gating of both CLC transporter subunits underlies voltage-dependent activation of the 2Cl-/1H+ exchanger ClC-7/Ostm1.The Journal of biological chemistry · 2013Article
- Regulatory phosphorylation induces extracellular conformational changes in a CLC anion channel.Biophysical journal · 2013Article
- CLC anion channel regulatory phosphorylation and conserved signal transduction domains.Biophysical journal · 2012Article
- Sequential interaction of chloride and proton ions with the fast gate steer the voltage-dependent gating in ClC-2 chloride channels.The Journal of physiology · 2012Article
- Partial least-squares functional mode analysis: application to the membrane proteins AQP1, Aqy1, and CLC-ec1.Biophysical journal · 2012Article
- CLC channels and transporters: proteins with borderline personalities.Biochimica et biophysica acta · 2010Review
- Antiport mechanism for Cl(-)/H(+) in ClC-ec1 from normal-mode analysis.Biophysical journal · 2010Article
- Voltage-dependent and -independent titration of specific residues accounts for complex gating of a ClC chloride channel by extracellular protons.The Journal of physiology · 2009Article
- Gating and trafficking of ClC-2 chloride channel without cystathionine beta-synthase domains.The Journal of physiology · 2008Article
- Myotonia-related mutations in the distal C-terminus of ClC-1 and ClC-0 chloride channels affect the structure of a poly-proline helix.The Biochemical journal · 2007Article
- Unique gating properties of C. elegans ClC anion channel splice variants are determined by altered CBS domain conformation and the R-helix linker.Channels (Austin, Tex.)Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional and structural studies demonstrate that Cl(-) channels of the ClC family have a dimeric double-barrelled structure, with each monomer contributing an identical pore. Studies with ClC-0, the prototype ClC channel, show the presence of independent mechanisms gating the individual pores or both pores simultaneously. A single-point mutation in the CBS-2 domain of ClC-0 has been shown to abolish slow gating. We have taken advantage of the high conservation of CBS domains in ClC channels to test for the presence of a slow gate in ClC-2 by reproducing this mutation (H811A). ClC-2-H811A showed faster opening kinetics and opened at more positive potentials than ClC-2. There was no difference in [Cl(-)](i) dependence. Additional neutralization of a putative pore gate glutamate side chain (E207V) abolished all gating. Resolving slow and fast gating relaxations, however, revealed that the H811A mutation affected both fast and slow gating processes in ClC-2. This suggests that slow and fast gating in ClC-2 are coupled, perhaps with slow gating contributing to the operation of the pore E207 as a protopore gate.
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