ArticleThe Journal of biological chemistry2006
The RACK1 homologue from Trypanosoma brucei is required for the onset and progression of cytokinesis.
Article in The Journal of biological chemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Cytosolic and Mitochondrial Hsp90 in Cytokinesis, Mitochondrial DNA Replication, and Drug Action in Trypanosoma brucei.Antimicrobial agents and chemotherapy · 2021Article
- Who Needs a Contractile Actomyosin Ring? The Plethora of Alternative Ways to Divide a Protozoan Parasite.Frontiers in cellular and infection microbiology · 2019Review
- WD40 Repeat Proteins: Signalling Scaffold with Diverse Functions.The protein journal · 2018Review
- A glutaredoxin in the mitochondrial intermembrane space has stage-specific functions in the thermo-tolerance and proliferation of African trypanosomes.Redox biology · 2018Article
- Functional analyses of the CIF1-CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis.Journal of cell science · 2017Article
- Scaffold proteins LACK and TRACK as potential drug targets in kinetoplastid parasites: Development of inhibitors.International journal for parasitology. Drugs and drug resistance · 2016Article
- RACK1 expression contributes to JNK activity, but JNK activity does not enhance RACK1 expression in hepatocellular carcinoma SMMC-7721 cells.Oncology letters · 2015Article
- LACK, a RACK1 ortholog, facilitates cytochrome c oxidase subunit expression to promote Leishmania major fitness.Molecular microbiology · 2015Article
- Unveiling the Trypanosoma cruzi Nuclear Proteome.PloS one · 2015Article
- Ribose 5-phosphate isomerase B knockdown compromises Trypanosoma brucei bloodstream form infectivity.PLoS neglected tropical diseases · 2015Article
- Maize ZmRACK1 is involved in the plant response to fungal phytopathogens.International journal of molecular sciences · 2014Article
- New insights into the molecular mechanisms of mitosis and cytokinesis in trypanosomes.International review of cell and molecular biology · 2014Review
- Distinct roles of a mitogen-activated protein kinase in cytokinesis between different life cycle forms of Trypanosoma brucei.Eukaryotic cell · 2014Article
- Cytokinesis in Trypanosoma brucei differs between bloodstream and tsetse trypomastigote forms: implications for microtubule-based morphogenesis and mutant analysis.Molecular microbiology · 2013Article
- Potent antitrypanosomal activities of heat shock protein 90 inhibitors in vitro and in vivo.The Journal of infectious diseases · 2013Article
- More than meets the eye: understanding Trypanosoma brucei morphology in the tsetse.Frontiers in cellular and infection microbiology · 2013Review
- Regulation of the cell division cycle in Trypanosoma brucei.Eukaryotic cell · 2012Review
- Receptor for activated C kinase ortholog of second-generation merozoite in Eimeria tenella: clone, characterization, and diclazuril-induced mRNA expression.Parasitology research · 2012Article
- RACK1, A multifaceted scaffolding protein: Structure and function.Cell communication and signaling : CCS · 2011Article
- Receptor for activated protein kinase C1 regulates cell proliferation by modulating calcium signaling.Hypertension (Dallas, Tex. : 1979) · 2011Article
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6 authors.
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Abstract
The receptor for activated C kinase 1 (RACK1) is a conserved scaffold protein that helps regulate a range of cell activities including cell growth, shape, and protein translation. We report that a homologue of RACK1 is required for cytokinesis in pathogenic Trypanosoma brucei. The protein, referred to as TRACK, is comprised of WD repeat elements and can complement cpc2 null mutants of Schizosaccharomyces pombe. TRACK is expressed throughout the trypanosome life cycle and is distributed predominantly in a perinuclear region and the cytoplasm but not along the endoplasmic reticulum, mitochondrion, or cleavage furrow of dividing cells. When tetracycline-inducible RNA interference (RNAi) is used to deplete the cellular content of TRACK, the cells remain metabolically active, but growth is inhibited. In bloodstream forms, growth arrest is due to a delay in the onset of cytokinesis. By contrast, procyclic forms are able to initiate cytokinesis in the absence of TRACK but arrest midway through cell cleavage. The RNAi cells undergo multiple rounds of partial cytokinesis and accumulate nuclei and cytoplasmic extensions with attached flagella. The TRACK RNAi construct is also inducible within infected mice. Under these conditions parasites are eliminated from peripheral blood within 3 days post-infection. Taken as a whole, these data indicate that trypanosomes utilize a RACK1 homologue to regulate the final stages of mitosis. Moreover, disrupting the interaction between TRACK and its partners might be targeted in the design of novel therapies.
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