ArticleBMC microbiology2006
Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay.
Article in BMC microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 177 papers, 1 of them a synthesis that pooled it.
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177 citing papers in PubMed, 1 synthesis or guideline pooled it, 402 citations in OpenAlex.
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- Genotyping of Coxiella burnetii isolates in Iran using Multiple-locus variable-number tandem repeat analysis (MLVA).BMC microbiology · 2025Article
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- Epidemiological Investigation of Animal Brucellosis in Domestic Ruminants in Greece from 2015 to 2022 and Genetic Characterization of Prevalent Strains.Pathogens (Basel, Switzerland) · 2024Article
- Analysis of the Brucella melitensis epidemic in Xinjiang: genotyping, polymorphism, antibiotic resistance and tracing.Annals of clinical microbiology and antimicrobials · 2024Article
- Evaluation of a Multilocus Variable-Number Tandem-Repeat Analysis Scheme for TypingMicroorganisms · 2024Article
- Molecular Epidemiological Study of a Human Brucellosis Outbreak - Weihai City, Shandong Province, China, 2022.China CDC weekly · 2024Article
- Genetic Diversity ofMicroorganisms · 2024Article
- Polyphasic Characterization ofMicroorganisms · 2024Article
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- Genotypic peculiarities of a human brucellosis case caused by Brucella suis biovar 5.Scientific reports · 2023Article
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- Genotype diversity of brucellosis agents isolated from humans and animals in Greece based on whole-genome sequencing.BMC infectious diseases · 2023Article
117 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe classification of Brucella into species and biovars relies on phenotypic characteristics and sometimes raises difficulties in the interpretation of the results due to an absence of standardization of the typing reagents. In addition, the resolution of this biotyping is moderate and requires the manipulation of the living agent. More efficient DNA-based methods are needed, and this work explores the suitability of multiple locus variable number tandem repeats analysis (MLVA) for both typing and species identification.
resultsEighty tandem repeat loci predicted to be polymorphic by genome sequence analysis of three available Brucella genome sequences were tested for polymorphism by genotyping 21 Brucella strains (18 reference strains representing the six 'classical' species and all biovars as well as 3 marine mammal strains currently recognized as members of two new species). The MLVA data efficiently cluster the strains as expected according to their species and biovar. For practical use, a subset of 15 loci preserving this clustering was selected and applied to the typing of 236 isolates. Using this MLVA-15 assay, the clusters generated correspond to the classical biotyping scheme of Brucella spp. The 15 markers have been divided into two groups, one comprising 8 user-friendly minisatellite markers with a good species identification capability (panel 1) and another complementary group of 7 microsatellite markers with higher discriminatory power (panel 2).
conclusionThe MLVA-15 assay can be applied to large collections of Brucella strains with automated or manual procedures, and can be proposed as a complement, or even a substitute, of classical biotyping methods. This is facilitated by the fact that MLVA is based on non-infectious material (DNA) whereas the biotyping procedure itself requires the manipulation of the living agent. The data produced can be queried on a dedicated MLVA web service site.
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