ArticleCurrent genetics2006
Agrobacterium T-DNA-mediated integration and gene replacement in the brown rot pathogen Monilinia fructicola.
Article in Current genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
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- YAP1 homologue-mediated redox sensing is crucial for a successful infection by Monilinia fructicola.Molecular plant pathology · 2017Article
- Function of the genetic element 'Mona' associated with fungicide resistance in Monilinia fructicola.Molecular plant pathology · 2017Article
- Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas.Planta · 2015Article
- Heterologous expression of the Monilinia fructicola CYP51 (MfCYP51) gene in Pichia pastoris confirms the mode of action of the novel fungicide, SYP-Z048.Frontiers in microbiology · 2015Article
- Article
- Horizontal gene transfer: sustaining pathogenicity and optimizing host-pathogen interactions.Molecular plant pathology · 2009Review
- Tissue specific response of Agrobacterium tumefaciens attachment to Sorghum bicolor (L) Moench.Physiology and molecular biology of plants : an international journal of functional plant biology · 2008Article
- Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles.Molecular biotechnology · 2008Article
- The cytochrome P450 lanosterol 14alpha-demethylase gene is a demethylation inhibitor fungicide resistance determinant in Monilinia fructicola field isolates from Georgia.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disruption in Monilinia fructicola, a fungal pathogen that causes brown rot disease of stone fruits. Transformation with a vector containing the neomycin phosphotransferase II (nptII) cassette flanked with 4 kb cutinase gene (Mfcut1) flanking sequences resulted in an average of 13 transformants per 10(5) spores. When assayed by PCR and DNA blot analyses, more than 50% of the transformants recovered had integrated in the targeted Mfcut1 locus. Both target-gene-specific and non-specific integrations carried direct (head-to-tail) repeat T-DNA integrations. Sequence analysis of these T-DNA integrations revealed that 26 bp of the T-DNA right border were missing at the junctions between direct repeats in all cases. The recombination event during non-specific T-DNA integration in this fungus was unlike that reported in Agrobacterium-mediated transformation in plants.
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