ArticleEukaryotic cell2006
The PbMDJ1 gene belongs to a conserved MDJ1/LON locus in thermodimorphic pathogenic fungi and encodes a heat shock protein that localizes to both the mitochondria and cell wall of Paracoccidioides brasiliensis.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 31 citations in OpenAlex.
- Heat Shock Proteins in Medically Relevant Fungal Pathogens: From Molecular Chaperones to Virulence Factors and Therapeutic Targets.Journal of fungi (Basel, Switzerland) · 2026Review
- Insights and Perspectives on the Role of Proteostasis and Heat Shock Proteins in Fungal Infections.Microorganisms · 2023Review
- Updates inJournal of fungi (Basel, Switzerland) · 2021Review
- Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei.Scientific reports · 2020Article
- The Human PathogenFrontiers in cellular and infection microbiology · 2020Article
- Extracellular Vesicle-Associated Transitory Cell Wall Components and Their Impact on the Interaction of Fungi with Host Cells.Frontiers in microbiology · 2016Review
- Intermolecular interactions of the malate synthase of Paracoccidioides spp.BMC microbiology · 2013Article
- Where do they come from and where do they go: candidates for regulating extracellular vesicle formation in fungi.International journal of molecular sciences · 2013Review
- Article
- Toward developing a universal treatment for fungal disease using radioimmunotherapy targeting common fungal antigens.Mycopathologia · 2012Article
- The pathogenic fungus Paracoccidioides brasiliensis exports extracellular vesicles containing highly immunogenic α-Galactosyl epitopes.Eukaryotic cell · 2011Article
- The paracoccidioides cell wall: past and present layers toward understanding interaction with the host.Frontiers in microbiology · 2011Article
- Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.PloS one · 2011Article
- Paracoccidioides brasiliensis enolase is a surface protein that binds plasminogen and mediates interaction of yeast forms with host cells.Infection and immunity · 2010Article
- The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin.BMC microbiology · 2009Article
- Extracellular vesicles produced by Cryptococcus neoformans contain protein components associated with virulence.Eukaryotic cell · 2008Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
J-domain (DnaJ) proteins, of the Hsp40 family, are essential cofactors of their cognate Hsp70 chaperones, besides acting as independent chaperones. In the present study, we have demonstrated the presence of Mdj1, a mitochondrial DnaJ member, not only in the mitochondria, where it is apparently sorted, but also in the cell wall of Paracoccidioides brasiliensis, a thermodimorphic pathogenic fungus. The molecule (PbMdj1) was localized to fungal yeast cells using both confocal and electron microscopy and also flow cytometry. The anti-recombinant PbMdj1 antibodies used in the reactions specifically recognized a single 55-kDa mitochondrial and cell wall (alkaline beta-mercaptoethanol extract) component, compatible with the predicted size of the protein devoid of its matrix peptide-targeting signal. Labeling was abundant throughout the cell wall and especially in the budding regions; however, anti-PbMdj1 did not affect fungal growth in the concentrations tested in vitro, possibly due to the poor access of the antibodies to their target in growing cells. Labeled mitochondria stood preferentially close to the plasma membrane, and gold particles were detected in the thin space between them, toward the cell surface. We show that Mdj1 and the mitochondrial proteinase Lon homologues are heat shock proteins in P. brasiliensis and that their gene organizations are conserved among thermodimorphic fungi and Aspergillus, where the genes are adjacent and have a common 5' region. This is the first time a DnaJ member has been observed on the cell surface, where its function is speculative.
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