ArticleClinical and vaccine immunology : CVI2006
Kinetics and diagnostic and prognostic potential of quantitative Western blot analysis and antigen-specific enzyme-linked immunosorbent assay in experimental canine leishmaniasis.
Article in Clinical and vaccine immunology : CVI, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Prognostic Value of Immunoglobulin G (IgG) Patterns by Western Blotting Immunodetection in Treated Dogs Previously Infected withVeterinary sciences · 2021Article
- Antibodies elicited by the CaniLeish® vaccine: long-term clinical follow-up study of dogs in Spain.Parasitology research · 2021Article
- Early antibody response and clinical outcome in experimental canine leishmaniasis.Scientific reports · 2019Article
- ELISA and western blotting for the detection of Hsp70 and Hsp83 antigens of Leishmania donovani.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2013Article
- Prevention of canine leishmaniosis in a hyper-endemic area using a combination of 10% imidacloprid/4.5% flumethrin.PloS one · 2013Article
- Dynamics and predictive potential of antibodies against insect-derived recombinant Leishmania infantum proteins during chemotherapy of naturally infected dogs.The American journal of tropical medicine and hygiene · 2010Article
- Leishmania tropica in rock hyraxes (Procavia capensis) in a focus of human cutaneous leishmaniasis.The American journal of tropical medicine and hygiene · 2010Article
- Canine leishmaniasis in Brazil: serological follow-up of a dog population in an endemic area of american visceral leishmaniasis.Journal of parasitology research · 2009Article
- Western Blot Analysis ofIranian journal of parasitologyArticle
- Detection of Potentially DiagnosticIranian journal of parasitologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative computerized Western blot analysis of antibody responses during experimental canine Leishmania infantum infection distinguished between immunodominant and nonimmunodominant protein bands. Six infected beagles, positive by both PCR and parasite culture, were monitored over 75 weeks postinfection and during a 12-week allopurinol treatment course. All dogs were symptomatic at the time of treatment. Of 12 antigenic bands examined, the immunodominant bands (12, 14, 24, 29, 48, and 68 kDa) showed significantly increased intensities (P<0.01) and higher frequencies of recognition than the nonimmunodominant bands at all time points. Detection of the former bands at 6 weeks postinfection preceded seroconversion by enzyme-linked immunosorbent assay (ELISA) both on crude Leishmania antigen or the recombinant proteins rK39 and HSP70. Reactivity with the 14-, 48-, and 68-kDa bands signified early infection, whereas increased reactivity with the 14-, 24-, and 29-kDa bands was associated with posttreatment parasite persistence and potential unfavorable prognosis. Total lane intensity (TLI) emerged as a sensitive marker for early infection and increased as early as 4 weeks postinfection. TLI had a significantly higher (P<0.01) relative increase rate than crude Leishmania antigen or HSP70 or rK39 ELISA at all time points. These immunodominant antigens and TLI, as determined by quantitative Western blotting, will be valuable for early detection and treatment evaluation of canine leishmaniasis.
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