ArticleProceedings of the National Academy of Sciences of the United States of America2006
In situ analysis of nitrogen fixation and metabolic switching in unicellular thermophilic cyanobacteria inhabiting hot spring microbial mats.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 200 citations in OpenAlex.
- Phototrophs trade growth for defense through graded regulatory states under escalating oxidative stress.iScience · 2026Article
- Response patterns of thermophilic Ascomycota to seasonal variations in Hammam Meskhoutine hot spring (Guelma, Algeria).Archives of microbiology · 2026Article
- Exploring the phylogeny, molecular and genomic adaptations of thermophilic microalgae and cyanobacteria.Planta · 2026Review
- Spatial scale modulates stochastic and deterministic influence on biogeography of photosynthetic biofilms in Southeast Asian hot springs.Environmental microbiome · 2025Article
- Abundant and active community members respond to diel cycles in hot spring phototrophic mats.The ISME journal · 2025Article
- Nitrogen-fixing Ability of a Thermophilic Sulfate-reducing Bacterium in the Genus Thermodesulfovibrio Isolated from a Terrestrial Hot Spring in Japan.Microbes and environments · 2025Article
- A unicellular cyanobacterium relies on sodium energetics to fix NNature communications · 2024Article
- Draft genome ofMicrobiology resource announcements · 2024Article
- Diurnal switches in diazotrophic lifestyle increase nitrogen contribution to cereals.Nature communications · 2023Article
- Community structure of thermophilic photosynthetic microbial mats and flocs at Sembawang Hot Spring, Singapore.Frontiers in microbiology · 2023Article
- Compensatory Transcriptional Response ofMolecules (Basel, Switzerland) · 2022Article
- Photosynthetic modulation during the diurnal cycle in a unicellular diazotrophic cyanobacterium grown under nitrogen-replete and nitrogen-fixing conditions.Scientific reports · 2022Article
- Article
- A Review of Cyanophage-Host Relationships: Highlighting Cyanophages as a Potential Cyanobacteria Control Strategy.Toxins · 2022Review
- Differential Phototactic Behavior of Closely Related Cyanobacterial Isolates from Yellowstone Hot Spring Biofilms.Applied and environmental microbiology · 2022Article
- Review
- Article
- Acclimation ofFrontiers in microbiology · 2022Article
- Molecular Components of Nitrogen Fixation Gene Cluster and Associated Enzymatic Activities of Non-Heterocystous Thermophilic CyanobacteriumLife (Basel, Switzerland) · 2021Article
- In-Situ Metatranscriptomic Analyses Reveal the Metabolic Flexibility of the Thermophilic Anoxygenic Photosynthetic BacteriumMicroorganisms · 2021Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Genome sequences of two Synechococcus ecotypes inhabiting the Octopus Spring microbial mat in Yellowstone National Park revealed the presence of all genes required for nitrogenase biosynthesis. We demonstrate that nif genes of the Synechococcus ecotypes are expressed in situ in a region of the mat that varies in temperature from 53.5 degrees C to 63.4 degrees C (average 60 degrees C); transcripts are only detected at the end of the day when the mat becomes anoxic. Nitrogenase activity in mat samples was also detected in the evening. Hitherto, N2 fixation in hot spring mats was attributed either to filamentous cyanobacteria (not present at >50 degrees C in these mats) or to heterotrophic bacteria. To explore how energy-generating processes of the Synechococcus ecotypes track natural light and O2 conditions, we evaluated accumulation of transcripts encoding proteins involved in photosynthesis, respiration, and fermentation. Transcripts from photosynthesis (cpcF, cpcE, psaB, and psbB) and respiration (coxA and cydA) genes declined in the evening. In contrast, transcripts encoding enzymes that may participate in fermentation fell into two categories; some (ldh, pdhB, ald, and ackA) decreased in the evening, whereas others (pflB, pflA, adhE, and acs) increased at the end of the day and remained high into the night. Energy required for N2 fixation during the night may be derived from fermentation pathways that become prominent as the mat becomes anoxic. In a broader context, our data suggest that there are critical regulatory switches in situ that are linked to the diel cycle and that these switches alter many metabolic processes within the microbial mat.
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