ArticleBMC nephrology2006
Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice.
Article in BMC nephrology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Cellular and Molecular Mechanisms of Kidney Development: From the Embryo to the Kidney Organoid.Frontiers in cell and developmental biology · 2020Review
- Insights from deconvolution of cell subtype proportions enhance the interpretation of functional genomic data.PloS one · 2019Article
- LHX3 is an advanced-stage prognostic biomarker and metastatic oncogene in hepatocellular carcinoma.Cancer biomarkers : section A of Disease markers · 2019Article
- Effects of Cyclosporine A on the Development of Metanephros in the Pregnant BALB/c Mice.Chinese medical journal · 2017Article
- LHX3 is an early stage and radiosensitivity prognostic biomarker in lung adenocarcinoma.Oncology reports · 2017Article
- Histone deacetylase (HDAC) activity is critical for embryonic kidney gene expression, growth, and differentiation.The Journal of biological chemistry · 2011Article
- The fate of Notch-deficient nephrogenic progenitor cells during metanephric kidney development.Kidney international · 2011Article
- Article
- Transcriptional control of terminal nephron differentiation.American journal of physiology. Renal physiology · 2008Review
- Transcriptional profiling of Wnt4 mutant mouse kidneys identifies genes expressed during nephron formation.Gene expression patterns : GEP · 2008Article
- Molecular insights into segmentation along the proximal-distal axis of the nephron.Journal of the American Society of Nephrology : JASN · 2007Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundLim1 is a homeobox gene that is essential for nephrogenesis. During metanephric kidney development, Lim1 is expressed in the nephric duct, ureteric buds, and the induced metanephric mesenchyme. Conditional ablation of Lim1 in the metanephric mesenchyme blocks the formation of nephrons at the nephric vesicle stage, leading to the production of small, non-functional kidneys that lack nephrons.
methodsIn the present study, we used Affymetrix probe arrays to screen for nephron-specific genes by comparing the expression profiles of control and Lim1 conditional mutant kidneys. Kidneys from two developmental stages, embryonic day 14.5 (E14.5) and 18.5 (E18.5), were examined.
resultsComparison of E18.5 kidney expression profiles generated a list of 465 nephron-specific gene candidates that showed a more than 2-fold increase in their expression level in control kidney versus the Lim1 conditional mutant kidney. Computational analysis confirmed that this screen enriched for kidney-specific genes. Furthermore, at least twenty-eight of the top fifty (56%) candidates (or their vertebrate orthologs) were previously reported to have a nephron-specific expression pattern. Our analysis of E14.5 expression data yielded 41 candidate genes that are up-regulated in the control kidneys compared to the conditional mutants. Three of them are related to the Notch signaling pathway that is known to be important in cell fate determination and nephron patterning.
conclusionTherefore, we demonstrate that Lim1 conditional mutant kidneys serve as a novel tissue source for comprehensive expression studies and provide a means to identify nephron-specific genes.
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