ArticleThe Journal of membrane biology2005
Characterization of regulatory volume behavior by fluorescence quenching in human corneal epithelial cells.
Article in The Journal of membrane biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
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- L-Carnitine Suppresses Transient Receptor Potential Vanilloid Type 1 Activation in Human Corneal Epithelial Cells.International journal of molecular sciences · 2023Article
- Identification of the chloride channel, leucine-rich repeat-containing protein 8, subfamily a (LRRC8A), in mouse cholangiocytes.Hepatology (Baltimore, Md.) · 2022Article
- The role of TRPV4 channels in ocular function and pathologies.Experimental eye research · 2020Review
- LRRC8 family proteins within lysosomes regulate cellular osmoregulation and enhance cell survival to multiple physiological stresses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Ions, the Movement of Water and the Apoptotic Volume Decrease.Frontiers in cell and developmental biology · 2020Review
- Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels.eLife · 2019Article
- LRRC8/VRAC anion channels enhance β-cell glucose sensing and insulin secretion.Nature communications · 2018Article
- Comparative Effects of Chloride Channel Inhibitors on LRRC8/VRAC-Mediated Chloride Conductance.Frontiers in pharmacology · 2017Article
- Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt-based anti-cancer drugs.The EMBO journal · 2015Article
- Detection of volume changes in calcein-stained cells using confocal microscopy.Journal of fluorescence · 2013Article
- cAMP-stimulated Cl- secretion is increased by glucocorticoids and inhibited by bumetanide in semicircular canal duct epithelium.BMC physiology · 2013Article
- Dependence of corneal epithelial cell proliferation on modulation of interactions between ERK1/2 and NKCC1.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2011Article
- Dependence of regulatory volume decrease on transient receptor potential vanilloid 4 (TRPV4) expression in human corneal epithelial cells.Cell calcium · 2008Article
- Functional and molecular characterization of multiple K-Cl cotransporter isoforms in corneal epithelial cells.Experimental eye research · 2007Article
- Differential dependence of regulatory volume decrease behavior in rabbit corneal epithelial cells on MAPK superfamily activation.Experimental eye research · 2007Article
- Roles of volume-activated Cl- currents and regulatory volume decrease in the cell cycle and proliferation in nasopharyngeal carcinoma cells.Cell proliferation · 2007Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
An in-depth understanding of the mechanisms underlying regulatory volume behavior in corneal epithelial cells has been in part hampered by the lack of adequate methodology for characterizing this phenomenon. Accordingly, we developed a novel approach to characterize time-dependent changes in relative cell volume induced by anisosmotic challenges in calcein-loaded SV40-immortalized human corneal epithelial (HCE) cells with a fluorescence microplate analyzer. During a hypertonic challenge, cells shrank rapidly, followed by a temperature-dependent regulatory volume increase (RVI), tau(c) = 19 min. In contrast, a hypotonic challenge induced a rapid (tau(c) = 2.5 min) regulatory volume decrease (RVD). Temperature decline from 37 to 24 degrees C reduced RVI by 59%, but did not affect RVD. Bumetanide (50 microM), ouabain (1 mM), DIDS (1 mM), EIPA (100 microM), or Na(+)-free solution reduced the RVI by 60, 61, 39, 32, and 69%, respectively. K+, Cl- channel and K(+)-Cl(-) cotransporter (KCC) inhibition obtained with either 4-AP (1 mM), DIDS (1 mM), DIOA (100 microM), high K+ (20 mM) or Cl(-)-free solution, suppressed RVD by 42, 47, 34, 52 and 58%, respectively. KCC activity also affects steady-state cell volume, since its inhibition or stimulation induced relative volume alterations under isotonic conditions. Taken together, K+ and Cl- channels in parallel with KCC activity are important mediators of RVD, whereas RVI is temperature-dependent and is essentially mediated by the Na(+)-K(+)-2Cl(-) cotransporter (Na(+)-K(+)-2Cl(-)) and the Na(+)-K(+) pump. Inhibition of K+ and Cl- channels and KCC but not Na(+)-K(+)-2Cl(-) affect steady-state cell volume under isotonic conditions. This is the first report that KCC activity is required for HCE cell volume regulation and maintenance of steady-state cell volume.
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