ArticleJournal of biomolecular techniques : JBT2005
Assessment of some tools for the characterization of the human osteoarthritic cartilage proteome.
Article in Journal of biomolecular techniques : JBT, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- A phase I-II controlled randomized trial using a promising novel cell-free formulation for articular cartilage regeneration as treatment of severe osteoarthritis of the knee.European journal of medical research · 2018Trial
- Elevated Serum Levels of YKL-40, YKL-39, and SI-CLP in Patients with Treatment Failure to DMARDs in Patients with Rheumatoid Arthritis.Biomedicines · 2024Article
- An integrative epi-transcriptomic approach identifies the human cartilage chitinase 3-like protein 2 (Epigenetics · 2022Article
- Biochemical Aspects of Scaffolds for Cartilage Tissue Engineering; from Basic Science to Regenerative Medicine.The archives of bone and joint surgery · 2022Review
- A promising novel formulation for articular cartilage regeneration: Preclinical evaluation of a treatment that produces SOX9 overexpression in human synovial fluid cells.Molecular medicine reports · 2018Article
- Subchondral bone proteomics in osteoarthritis: Current status and perspectives.Journal of orthopaedic translation · 2015Review
- Structural and thermodynamic insights into chitooligosaccharide binding to human cartilage chitinase 3-like protein 2 (CHI3L2 or YKL-39).The Journal of biological chemistry · 2015Article
- Proteomic Analysis of Engineered Cartilage.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Comparative proteomic analysis of hypertrophic chondrocytes in osteoarthritis.Clinical proteomics · 2015Article
- Genomic analysis and differential expression of HMG and S100A family in human arthritis: upregulated expression of chemokines, IL-8 and nitric oxide by HMGB1.DNA and cell biology · 2014Article
- Absolute quantification of selected proteins in the human osteoarthritic secretome.International journal of molecular sciences · 2013Article
- Carprofen inhibits the release of matrix metalloproteinases 1, 3, and 13 in the secretome of an explant model of articular cartilage stimulated with interleukin 1β.Arthritis research & therapy · 2013Article
- Biochemical and proteomic characterization of alkaptonuric chondrocytes.Journal of cellular physiology · 2012Article
- Development of an ELISA-array for simultaneous detection of five encephalitis viruses.Virology journal · 2012Article
- Proteomic analysis of Col11a1-associated protein complexes.Proteomics · 2011Article
- Expression of Osteoarthritis Marker YKL-39 is Stimulated by Transforming Growth Factor Beta (TGF-beta) and IL-4 in Differentiating Macrophages.Biomarker insights · 2008Article
- Human chitinases and chitinase-like proteins as indicators for inflammation and cancer.Biomarker insights · 2007Article
- Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes.Arthritis research & therapy · 2007Article
- A systems biology approach to synovial joint lubrication in health, injury, and disease.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the proteome of osteoarthritic articular cartilage has been poorly investigated as yet, we adapted proteomic technologies to the study of the proteins secreted or released by fresh human osteoarthritic cartilage in culture. Fresh cartilage explants were obtained from three donors undergoing surgery for knee joint replacement. The explants were dissected out, minced, and incubated in serum-free culture medium. After 48 h, proteins in the medium were identified by two-dimensional or off-gel electrophoresis coupled to tandem mass spectrometry, or by using an antibody-based protein microarray designed to detect angiogenic factors, growth factors, chemokines, and cytokines. We identified a series of 43 proteins. Some of these proteins were already described as secretion products of chondrocytes, such as YKL-39 or osteoprotegerin, while several other were known proteins but have never been reported previously in cartilage, such as the serum amyloid P-component, the vitamin D binding protein, the pigment epithelium derived factor, the pulmonary and activation-regulated chemokine, lyl-1, thrombopoietin, fibrinogen, angiogenin, gelsolin, and osteoglycin/mimecan. While this study enabled the identification of novel proteins secreted or released by human osteoarthritic cartilage, the goal of the present work was essentially to describe the technical approach necessary for a systematic study of osteoarthritic cartilages from a large population of donors, in order to be able to select the good markers and/or targets for this poorly explored disease.
Indexed as
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16461950PMC2291736W2420849What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.