ArticleProceedings of the National Academy of Sciences of the United States of America2006
13C isotopologue perturbation studies of Listeria monocytogenes carbon metabolism and its modulation by the virulence regulator PrfA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 94 citations in OpenAlex.
- Trehalose metabolism and its impact on PrfA activity inJournal of bacteriology · 2026Article
- Transcriptional responses of Listeria monocytogenes to low-temperature stress reveal distinct adaptive mechanisms in the absence of BKD and PrfA activation.Microbial pathogenesis · 2025Article
- How Bacterial Pathogens Coordinate Appetite with Virulence.Microbiology and molecular biology reviews : MMBR · 2023Review
- Article
- Metabolism of the Gram-Positive Bacterial PathogenMicrobiology spectrum · 2019Review
- Review
- Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni.PLoS biology · 2017Article
- Complete Circular Genome Sequence and Temperature Independent Adaptation to Anaerobiosis ofFrontiers in microbiology · 2017Article
- Systems Level Analyses Reveal Multiple Regulatory Activities of CodY Controlling Metabolism, Motility and Virulence in Listeria monocytogenes.PLoS genetics · 2016Article
- Adaptation inFrontiers in microbiology · 2016Review
- Regulating the Intersection of Metabolism and Pathogenesis in Gram-positive Bacteria.Microbiology spectrum · 2015Review
- Characterization of the pivotal carbon metabolism of Streptococcus suis serotype 2 under ex vivo and chemically defined in vitro conditions by isotopologue profiling.The Journal of biological chemistry · 2015Article
- Analysis of carbon substrates used by Listeria monocytogenes during growth in J774A.1 macrophages suggests a bipartite intracellular metabolism.Frontiers in cellular and infection microbiology · 2014Article
- 13C-flux spectral analysis of host-pathogen metabolism reveals a mixed diet for intracellular Mycobacterium tuberculosis.Chemistry & biology · 2013Article
- Functional γ-Aminobutyrate Shunt in Listeria monocytogenes: role in acid tolerance and succinate biosynthesis.Applied and environmental microbiology · 2013Article
- MudPIT profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of Listeria monocytogenes EGD-e.PloS one · 2013Article
- Metabolic adaptation and protein complexes in prokaryotes.Metabolites · 2012Article
- Integrative genomic analysis identifies isoleucine and CodY as regulators of Listeria monocytogenes virulence.PLoS genetics · 2012Article
- Bactericidal antibiotics do not appear to cause oxidative stress in Listeria monocytogenes.Applied and environmental microbiology · 2012Article
- Characterization of central carbon metabolism of Streptococcus pneumoniae by isotopologue profiling.The Journal of biological chemistry · 2012Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The carbon metabolism of Listeria monocytogenes (Lm) EGD and the two isogenic mutant strains LmDeltaprfA and LmDeltaprfApPRFA* (showing no or enhanced expression, respectively, of the virulence factor PrfA) was determined by 13C isotopologue perturbation. After growth of the bacteria in a defined medium containing a mixture of [U-13C6]glucose and glucose with natural 13C abundance (1:25, wt/wt), 14 amino acids were isolated and analyzed by NMR spectroscopy. Multiply 13C-labeled isotopologues were determined quantitatively by signal deconvolution. The 13C enrichments and isotopologue patterns allowed the reconstruction of most amino acid biosynthesis pathways and illustrated that overproduced PrfA may strongly influence the synthesis of some amino acids, notably that of the branched amino acids (Val, Ile, and Leu). Retrobiosynthetic analysis of the isotopologue compositions showed that degradation of glucose occurs to a large extent via the pentose phosphate pathway and that the citrate cycle is incomplete because of the absence of 2-oxoglutarate dehydrogenase activity. The reconstructed labeling pattern of oxaloacetate indicated its formation by carboxylation of pyruvate. This metabolic reaction seems to have a strong impact on the growth requirement in defined minimal medium. Bioinformatical steady-state network analyses and flux distribution predictions confirmed the experimental data and predicted metabolite fluxes through the enzymes of the pathways under study.
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