ArticleApplied and environmental microbiology2006
Temperature-regulated formation of mycelial mat-like biofilms by Legionella pneumophila.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 113 citations in OpenAlex.
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Legionella Survives and Elongates in Algal Consortia Containing Bacteria in Alkaline Oligotrophic Conditions.Microbes and environments · 2025Article
- Review
- Dynamics of drinking water biofilm formation associated with Legionella spp. colonization.NPJ biofilms and microbiomes · 2024Article
- Legionella colonization and 3D spatial location within a Pseudomonas biofilm.Scientific reports · 2024Article
- Ecology ofBiofilm · 2024Review
- Sodium levels and grazing pressure shape natural communities of the intracellular pathogen Legionella.Microbiome · 2023Article
- Characterization of a Novel Regulator of Biofilm Formation in the PathogenBiomolecules · 2022Article
- Decreasing Pasteurization Treatment Efficiency against Amoeba-GrownInternational journal of environmental research and public health · 2022Article
- Differential Bacterial Predation by Free-Living Amoebae May Result in Blooms ofMicroorganisms · 2021Article
- Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens.NPJ biofilms and microbiomes · 2020Article
- Small Rho GTPases and the Effector VipA Mediate the Invasion of Epithelial Cells by FilamentousFrontiers in cellular and infection microbiology · 2018Article
- Factors Mediating Environmental Biofilm Formation byFrontiers in cellular and infection microbiology · 2018Review
- Article
- Intra-Species and Inter-Kingdom Signaling ofFrontiers in microbiology · 2017Review
- The Flagellar Regulon ofFrontiers in cellular and infection microbiology · 2017Review
- The Legionella pneumophila GIG operon responds to gold and copper in planktonic and biofilm cultures.PloS one · 2017Article
- Iron Availability Modulates the Persistence of Legionella pneumophila in Complex Biofilms.Microbes and environments · 2016Article
- Response of Vibrio cholerae to Low-Temperature Shifts: CspV Regulation of Type VI Secretion, Biofilm Formation, and Association with Zooplankton.Applied and environmental microbiology · 2016Article
- Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fifty strains representing 38 species of the genus Legionella were examined for biofilm formation on glass, polystyrene, and polypropylene surfaces in static cultures at 25 degrees C, 37 degrees C, and 42 degrees C. Strains of Legionella pneumophila, the most common causative agent of Legionnaires' disease, were found to have the highest ability to form biofilms among the test strains. The quantity, rate of formation, and adherence stability of L. pneumophila biofilms showed considerable dependence on both temperature and surface material. Glass and polystyrene surfaces gave between two- to sevenfold-higher yields of biofilms at 37 degrees C or 42 degrees C than at 25 degrees C; conversely, polypropylene surface had between 2 to 16 times higher yields at 25 degrees C than at 37 degrees C or 42 degrees C. On glass surfaces, the biofilms were formed faster but attached less stably at 37 degrees C or 42 degrees C than at 25 degrees C. Both scanning electron microscopy and confocal laser scanning microscopy revealed that biofilms formed at 37 degrees C or 42 degrees C were mycelial mat like and were composed of filamentous cells, while at 25 degrees C, cells were rod shaped. Planktonic cells outside of biofilms or in shaken liquid cultures were rod shaped. Notably, the filamentous cells were found to be multinucleate and lacking septa, but a recA null mutant of L. pneumophila was unaffected in its temperature-regulated filamentation within biofilms. Our data also showed that filamentous cells were able to rapidly give rise to a large number of short rods in a fresh liquid culture at 37 degrees C. The possibility of this biofilm to represent a novel strategy by L. pneumophila to compete for proliferation among the environmental microbiota is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.