ArticleApplied and environmental microbiology2006
Molecular assays for detecting Aphanomyces invadans in ulcerative mycotic fish lesions.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- In vitro assessment of the antimicrobial efficacy of chitosan nanoparticles against major fish pathogens and their cytotoxicity to fish cell lines.Journal of fish diseases · 2020Article
- Epizootic ulcerative syndrome - First report of evidence from South Africa's largest and premier conservation area, the Kruger National Park.International journal for parasitology. Parasites and wildlife · 2019Article
- Editing the genome of Aphanomyces invadans using CRISPR/Cas9.Parasites & vectors · 2018Article
- Entomophthoromycosis and mucormycosis as causes of pneumonia in Vietnamese potbellied pigs.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Peptide nucleic acids: Advanced tools for biomedical applications.Journal of biotechnology · 2017Review
- qPCR assays forPhycologia · 2017Article
- Emerging oomycete threats to plants and animals.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Synthesis and Evaluation of Novel Oxyalkylated Derivatives of 2',4'-Dihydroxychalcone as Anti-Oomycete Agents against Bronopol Resistant Strains of Saprolegnia sp.International journal of molecular sciences · 2016Article
- Current ecological understanding of fungal-like pathogens of fish: what lies beneath?Frontiers in microbiology · 2014Review
- Article
- Development and standardization of a monoclonal antibody-based rapid flow-through immunoassay for the detection of Aphanomyces invadans in the field.Journal of veterinary science · 2013Article
- Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci.BMC microbiology · 2009Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathogenic oomycete Aphanomyces invadans is the primary etiological agent in ulcerative mycosis, an ulcerative skin disease caused by a fungus-like agent of wild and cultured fish. We developed sensitive PCR and fluorescent peptide nucleic acid in situ hybridization (FISH) assays to detect A. invadans. Laboratory-challenged killifish (Fundulus heteroclitus) were first tested to optimize and validate the assays. Skin ulcers of Atlantic menhaden (Brevoortia tyrannus) from populations found in the Pamlico and Neuse River estuaries in North Carolina were then surveyed. Results from both assays indicated that all of the lesioned menhaden (n = 50) collected in September 2004 were positive for A. invadans. Neither the FISH assay nor the PCR assay cross-reacted with other closely related oomycetes. These results provided strong evidence that A. invadans is the primary oomycete pathogen in ulcerative mycosis and demonstrated the utility of the assays. The FISH assay is the first molecular assay to provide unambiguous visual confirmation that hyphae in the ulcerated lesions were exclusively A. invadans.
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