ArticleApplied and environmental microbiology2006
Degradation of 4-chloro-2-methylphenoxyacetic acid in top- and subsoil is quantitatively linked to the class III tfdA gene.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Interspecies Exchange of Mobile Genetic Elements During a Plant Disease Outbreak.Genome biology and evolution · 2026Article
- Modified zeolite-supported biofilm in service of pesticide biodegradation.Environmental science and pollution research international · 2021Article
- Biodegradation of Pesticides at the Limit: Kinetics and Microbial Substrate Use at Low Concentrations.Frontiers in microbiology · 2020Article
- Photodegradation using TiOEnvironmental science and pollution research international · 2019Article
- Article
- Diversity of bacterial community during ensiling and subsequent exposure to air in whole-plant maize silage.Asian-Australasian journal of animal sciences · 2018Article
- Microbial changes linked to the accelerated degradation of the herbicide atrazine in a range of temperate soils.Environmental science and pollution research international · 2017Article
- Functional Redundancy of Linuron Degradation in Microbial Communities in Agricultural Soil and Biopurification Systems.Applied and environmental microbiology · 2016Article
- Evidence for the importance of litter as a co-substrate for MCPA dissipation in an agricultural soil.Environmental science and pollution research international · 2016Article
- Cloning, expression, characterization and mutational analysis of the tfdA gene from Cupriavidus campinensis BJ71.World journal of microbiology & biotechnology · 2015Article
- 16S rRNA gene phylogeny and tfdA gene analysis of 2,4-D-degrading bacteria isolated in China.World journal of microbiology & biotechnology · 2014Article
- Modeling of phenoxy acid herbicide mineralization and growth of microbial degraders in 15 soils monitored by quantitative real-time PCR of the functional tfdA gene.Applied and environmental microbiology · 2012Article
- The earthworm Aporrectodea caliginosa stimulates abundance and activity of phenoxyalkanoic acid herbicide degraders.The ISME journal · 2011Article
- Relative and absolute quantitative real-time PCR-based quantifications of hcnC and phlD gene transcripts in natural soil spiked with Pseudomonas sp. strain LBUM300.Applied and environmental microbiology · 2011Article
- Abundance of novel and diverse tfdA-like genes, encoding putative phenoxyalkanoic acid herbicide-degrading dioxygenases, in soil.Applied and environmental microbiology · 2010Article
- TaqMan probe-based real-time PCR assay for detection and discrimination of class I, II, and III tfdA genes in soils treated with phenoxy acid herbicides.Applied and environmental microbiology · 2009Article
- Detection and quantification of Geobacter lovleyi strain SZ: implications for bioremediation at tetrachloroethene- and uranium-impacted sites.Applied and environmental microbiology · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tfdA gene is known to be involved in the first step of the degradation of the phenoxy acid herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) in several soil bacteria, but bacteria containing other tfdA-like genes have been isolated as well. A quantitative real-time PCR method was used to monitor the increase in the concentration of tfdA genes during degradation of MCPA in sandy topsoil and subsoil over a period of 115 days. Quantitative PCR revealed growth in the tfdA-containing bacterial community, from 500 genes g(-1) soil to approximately 3 x 10(4) genes g(-1) soil and to 7 x 10(5) genes g(-1) soil for topsoil initially added to 2.3 mg MCPA kg(-1) (dry weight) soil and 20 mg MCPA kg(-1) (dry weight) soil, respectively. We analyzed the diversity of the tfdA gene during the degradation experiment. Analyses of melting curves of real-time PCR amplification products showed that a shift in the dominant tfdA population structure occurred during the degradation period. Further denaturing gradient gel electrophoresis and sequence analysis revealed that the tfdA genes responsible for the degradation of MCPA belonged to the class III tfdA genes, while the tfdA genes present in the soil before the occurrence of degradation belonged to the class I tfdA genes. The implications of these results is that the initial assessment of functional genes in soils does not necessarily reflect the organisms or genes that would carry out the degradation of the compounds in question.
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