ArticleApplied and environmental microbiology2006
Identification of an endo-beta-1,4-D-xylanase from Magnaporthe grisea by gene knockout analysis, purification, and heterologous expression.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 58 citations in OpenAlex.
- Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus.Rice (New York, N.Y.) · 2022Article
- Comparative Transcriptomics and Gene Knockout Reveal Virulence Factors ofFrontiers in microbiology · 2022Article
- Article
- Cytotoxic function of xylanase VdXyn4 in the plant vascular wilt pathogen Verticillium dahliae.Plant physiology · 2021Article
- Global Characterization of GH10 Family Xylanase Genes inInternational journal of molecular sciences · 2020Article
- Two genes encoding GH10 xylanases are essential for the virulence of the oomycete plant pathogen Phytophthora parasitica.Current genetics · 2018Article
- Deletion of Endo-β-1,4-XylanaseFrontiers in plant science · 2018Article
- Phylogenetic analysis of β-xylanase SRXL1 of Sporisorium reilianum and its relationship with families (GH10 and GH11) of Ascomycetes and Basidiomycetes.Scientific reports · 2016Article
- Disruption of the Gene Encoding Endo-β-1, 4-Xylanase Affects the Growth and Virulence ofFrontiers in microbiology · 2016Article
- Secreted Alpha-N-Arabinofuranosidase B Protein Is Required for the Full Virulence of Magnaporthe oryzae and Triggers Host Defences.PloS one · 2016Article
- A high-throughput gene disruption methodology for the entomopathogenic fungus Metarhizium robertsii.PloS one · 2014Article
- Fungal plant cell wall-degrading enzyme database: a platform for comparative and evolutionary genomics in fungi and Oomycetes.BMC genomics · 2013Article
- De novo transcriptome sequencing and analysis for Venturia inaequalis, the devastating apple scab pathogen.PloS one · 2013Article
- The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots.Molecular plant pathology · 2011Article
- Whole-genome sequence of the xylanase-producing Mesoflavibacter zeaxanthinifaciens strain S86.Journal of bacteriology · 2011Article
- Interaction transcriptome analysis identifies Magnaporthe oryzae BAS1-4 as Biotrophy-associated secreted proteins in rice blast disease.The Plant cell · 2009Article
- Biochemical and molecular characterization of a putative endoglucanase in Magnaporthe grisea.Current genetics · 2008Article
- Xyr1 regulates xylanase but not cellulase formation in the head blight fungus Fusarium graminearum.Current genetics · 2007Article
- Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola.Journal of Zhejiang University. Science. BArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Magnaporthe grisea, a destructive ascomycetous pathogen of rice, secretes cell wall-degrading enzymes into a culture medium containing purified rice cell walls as the sole carbon source. From M. grisea grown under the culture conditions described here, we have identified an expressed sequenced tag, XYL-6, a gene that is also expressed in M. grisea-infected rice leaves 24 h postinoculation with conidia. This gene encodes a protein about 65% similar to endo-beta-1,4-D-glycanases within glycoside hydrolase family GH10. A M. grisea knockout mutant for XYL-6 was created, and it was shown to be as virulent as the parent strain in infecting the rice host. The proteins secreted by the parent strain and by the xyl-6Delta mutant were each fractionated by liquid chromatography, and the collected fractions were assayed for endo-beta-1,4-D-glucanase or endo-beta-1,4-D-xylanase activities. Two protein-containing peaks with endo-beta-1,4-D-xylanase activity secreted by the parent strain are not detectable in the column eluant of the proteins secreted by the mutant. The two endoxylanases (XYL-6alpha and XYL-6beta) from the parent were each purified to homogeneity. N-terminal amino acid sequencing indicated that XYL-6alpha is a fragment of XYL-6beta and that XYL-6beta is identical to the deduced protein sequence encoded by the XYL-6 gene. Finally, XYL-6 was introduced into Pichia pastoris for heterologous expression, which resulted in the purification of a fusion protein, XYL-6H, from the Pichia pastoris culture filtrate. XYL-6H is active in cleaving arabinoxylan. These experiments unequivocally established that the XYL-6 gene encodes a secreted endo-beta-1,4-D-xylanase.
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