ArticleThe Plant cell2006
High-resolution single-copy gene fluorescence in situ hybridization and its use in the construction of a cytogenetic map of maize chromosome 9.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- DNA content, repeatome composition and origin of the Zea mays micronuclei.Scientific reports · 2025Article
- Isolation of Meiocytes and Cytological Analyses of Male Meiotic Chromosomes in Soybean, Lettuce, and Maize.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Development and applications of a collection of single copy gene-based cytogenetic DNA markers in garden asparagus.Frontiers in plant science · 2022Article
- A Dual-Color Tyr-FISH Method for Visualizing Genes/Markers on Plant Chromosomes to Create Integrated Genetic and Cytogenetic Maps.International journal of molecular sciences · 2021Article
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- A universal chromosome identification system for maize and wild Zea species.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- A Molecular Cytogenetic Map of Scallop (Patinopecten yessoensis).Marine biotechnology (New York, N.Y.) · 2019Article
- ZmRAD51C is Essential for Double-Strand Break Repair and Homologous Recombination in Maize Meiosis.International journal of molecular sciences · 2019Article
- Meiotic crossovers characterized by haplotype-specific chromosome painting in maize.Nature communications · 2019Article
- Whole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationships.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Location of low copy genes in chromosomes of Brachiaria spp.Molecular biology reports · 2018Article
- Mysterious meiotic behavior of autopolyploid and allopolyploid maize.Comparative cytogenetics · 2018Article
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- Updating the maize karyotype by chromosome DNA sizing.PloS one · 2018Article
- Highly distinct chromosomal structures in cowpea (Vigna unguiculata), as revealed by molecular cytogenetic analysis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
- The Axial Element Protein DESYNAPTIC2 Mediates Meiotic Double-Strand Break Formation and Synaptonemal Complex Assembly in Maize.The Plant cell · 2015Article
- High resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of Rosa.BMC genetics · 2015Article
- Construction of cytogenetic map of Gossypium herbaceum chromosome 1 and its integration with genetic maps.Molecular cytogenetics · 2015Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize genetically mapped genes onto chromosomes by fluorescence in situ hybridization (FISH). Detection of single-copy genes on plant chromosomes has been difficult. In this study, we developed a squash FISH procedure allowing successful detection of single-copy genes on maize (Zea mays) pachytene chromosomes. Using this method, the shortest probe that can be detected is 3.1 kb, and two sequences separated by approximately 100 kb can be resolved. To show the robust nature of this protocol, we localized nine genetically mapped single-copy genes on chromosome 9 in one FISH experiment. Integration of existing information from genetic maps and the BAC contig-based physical map with the cytological structure of chromosome 9 provides a comprehensive cross-referenced cytogenetic map and shows the dramatic reduction of recombination in the pericentromeric heterochromatic region. To establish a feasible mapping system for maize, we also developed a probe cocktail for unambiguous identification of the 10 maize pachytene chromosomes. These results provide a starting point toward constructing a high-resolution integrated cytogenetic map of maize.
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