ArticleThe Plant cell2006
Vacuolar H+-ATPase activity is required for endocytic and secretory trafficking in Arabidopsis.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 436 papers.
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Who cites it
436 citing papers in PubMed, 852 citations in OpenAlex.
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- Cisternal Maturation of the Golgi Apparatus in Budding Yeast.Sub-cellular biochemistry · 2026Review
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- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- Association of Golgi Stacks and Recycling Endosomes Ensures Post-Golgi Trafficking.Sub-cellular biochemistry · 2026Review
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- Spatiotemporal dynamics of AHA2 reveal that RALF1 induces its endocytosis and vacuolar degradation.Plant communications · 2025Article
- Concanamycins Are Key Contributors to the Virulence of the Potato Common Scab Pathogen Streptomyces scabiei.Molecular plant pathology · 2025Article
- Retrieval from vacuolar and endosomal compartments underpinning the neofunctionalization of SNARE in plants.Nature plants · 2025Article
- HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane.Nature communications · 2025Article
- Detection of Cytosolic Ion Concentrations by the Trans-Golgi Network/Early Endosome is Important for Salt Tolerance.bioRxiv : the preprint server for biology · 2025Article
- Translational fidelity and longevity are genetically linked.Nature communications · 2025Article
- Cytosolic- and membrane-localized oxidized indole-3-acetic acid formation regulates developmental auxin transients.Plant physiology · 2025Article
376 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotic cells, compartments of the highly dynamic endomembrane system are acidified to varying degrees by the activity of vacuolar H(+)-ATPases (V-ATPases). In the Arabidopsis thaliana genome, most V-ATPase subunits are encoded by small gene families, thus offering potential for a multitude of enzyme complexes with different kinetic properties and localizations. We have determined the subcellular localization of the three Arabidopsis isoforms of the membrane-integral V-ATPase subunit VHA-a. Colocalization experiments as well as immunogold labeling showed that VHA-a1 is preferentially found in the trans-Golgi network (TGN), the main sorting compartment of the secretory pathway. Uptake experiments with the endocytic tracer FM4-64 revealed rapid colocalization with VHA-a1, indicating that the TGN may act as an early endosomal compartment. Concanamycin A, a specific V-ATPase inhibitor, blocks the endocytic transport of FM4-64 to the tonoplast, causes the accumulation of FM4-64 together with newly synthesized plasma membrane proteins, and interferes with the formation of brefeldin A compartments. Furthermore, nascent cell plates are rapidly stained by FM4-64, indicating that endocytosed material is redirected into the secretory flow after reaching the TGN. Together, our results suggest the convergence of the early endocytic and secretory trafficking pathways in the TGN.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.