ArticleJournal of clinical pathology2006
Phosphohistone H3 labelling for histoprognostic grading of breast adenocarcinomas and computer-assisted determination of mitotic index.
Article in Journal of clinical pathology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 67 citations in OpenAlex.
- Association of baseline tumor-infiltrating lymphocytes and cell-cycle regulation markers on prognosis and mortality in patients with advanced breast cancer according to tumor characteristics and treatment type: an observational study.Breast cancer research and treatment · 2026Observational
- Phospho-Histone H3 as a Tool for Improved Mitotic Counting and Risk Assessment in Gastrointestinal Stromal Tumors: A Study of 241 Cases.Medical science monitor : international medical journal of experimental and clinical research · 2026Article
- Expression of phosphohistone H3 and X-linked inhibitors of apoptosis-related proteins in mucoepidermoid carcinoma and adenoid cystic carcinoma of salivary glands.Scientific reports · 2025Article
- Article
- The Utility of Phosphohistone H3 in Inter-Observer Variability of Mitotic Count in Meningioma, is There Any Benefit?Asian Pacific journal of cancer prevention : APJCP · 2021Article
- Assessment of tumour proliferation by use of the mitotic activity index, and Ki67 and phosphohistone H3 expression, in early-stage luminal breast cancer.Histopathology · 2020Observational
- Prognostic and Clinicopathological Correlations of Cell Cycle Marker Expressions before and after the Primary Systemic Therapy of Breast Cancer.Pathology oncology research : POR · 2020Article
- Phosphohistone H3 (PHH3) as a surrogate of mitotic figure count for grading in meningiomas: a comparison of PHH3 (S10) versus PHH3 (S28) antibodies.Virchows Archiv : an international journal of pathology · 2019Article
- Pooling analysis on prognostic value of PHH3 expression in cancer patients.Cancer management and research · 2018Article
- The value of phosphohistone H3 as a proliferation marker for evaluating invasive breast cancers: A comparative study with Ki67.Oncotarget · 2017Article
- Association Between Phosphorylated Histone H3 and Oncotype DX Recurrence Scores in Breast Cancer.Applied immunohistochemistry & molecular morphology : AIMM · 2017Article
- Can PPH3 be helpful to assess the discordant grade in primary and metastatic enteropancreatic neuroendocrine tumors?Endocrine · 2016Article
- Expression of cell cycle markers is predictive of the response to primary systemic therapy of locally advanced breast cancer.Virchows Archiv : an international journal of pathology · 2016Article
- Better see to better agree: phosphohistone H3 increases interobserver agreement in mitotic count for meningioma grading and imposes new specific thresholds.Neuro-oncology · 2015Article
- PHH3 as an Ancillary Mitotic Marker in Gastrointestinal Stromal Tumors.Journal of pathology and translational medicine · 2015Article
- Value of counting positive PHH3 cells in the diagnosis of uterine smooth muscle tumors.International journal of clinical and experimental pathology · 2015Article
- Detection of mitotic figures and G2+ tumor nuclei with histone markers correlates with worse overall survival in patients with Merkel cell carcinoma.Journal of cutaneous pathology · 2014Article
- Garbage in, garbage out: a critical evaluation of strategies used for validation of immunohistochemical biomarkers.Molecular oncology · 2014Review
- Current breast cancer proliferative markers correlate variably based on decoupled duration of cell cycle phases.Scientific reports · 2014Article
- Chromatin CKAP2, a new proliferation marker, as independent prognostic indicator in breast cancer.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroscopic evaluation of mitotic figures is a routine procedure in the assessment of the histoprognostic grade of tumours. Nevertheless, their count may be fraught with difficulties. As histone H3 phosphorylation at serine 10 is closely linked to chromosomal condensation, a new monoclonal antibody directed to phosphorylated histone H3 (PPH3) was recently proposed to detect mitotic cells.
aimTo test the reliability of this antibody in detecting and counting mitotic figures in sections of breast adenocarcinomas, because of the importance of mitotic count in histoprognostic grading.
methodsThe pattern of PPH3 staining in formalin-fixed paraffin wax-embedded tissues, including normal tissues and a series of 39 breast adenocarcinomas, was examined. A new computer-assisted method was also developed for determining the mitotic index. RESULTS AND
conclusionsIn all tissues tested, PPH3-labelled mitotic figures were easily detected, allowing a rapid identification of the area of highest mitotic activity. In breast carcinomas, a strong correlation was observed between PPH3-stained and haematoxylin and eosin-stained mitotic counts (r = 0.86, p<0.0001). Counting of prophase nuclei that coexpress cyclin B1, a marker of the G2/M phase, was possible by PPH3 staining; its accuracy led us to reconsider the tumour grade in three cases. Finally, an automatic computer-assisted method was designed for assessing mitotic index with confocal microscopy and image-analysis software.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.