ArticleBiophysical journal2006
Domain-induced activation of human phospholipase A2 type IIA: local versus global lipid composition.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 74 citations in OpenAlex.
- Article
- Structural aspects and activation mechanism of human secretory group IIA phospholipase.European biophysics journal : EBJ · 2020Article
- Fluorescent Lipo-Beads for the Sensitive Detection of Phospholipase AACS biomaterials science & engineering · 2020Article
- Enzyme-Responsive Liposomes for the Delivery of Anticancer Drugs.Bioconjugate chemistry · 2017Review
- Development of a cell-based bioassay for phospholipase A2-triggered liposomal drug release.PloS one · 2015Article
- High ω-3:ω-6 fatty acids ratio increases fatty acid binding protein 4 and extracellular secretory phospholipase A2IIa in human ectopic endometrial cells.Iranian journal of reproductive medicine · 2014Article
- Accumulated bending energy elicits neutral sphingomyelinase activity in human red blood cells.Biophysical journal · 2012Article
- Secretory phospholipase A₂ responsive liposomes.Journal of pharmaceutical sciences · 2011Article
- Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains.Journal of lipid research · 2011Article
- Importance of residue 13 and the C-terminus for the structure and activity of the antimicrobial peptide aurein 2.2.Biophysical journal · 2010Article
- Enzyme-triggered nanomedicine: drug release strategies in cancer therapy.Molecular membrane biology · 2010Review
- Influence of lipid heterogeneity and phase behavior on phospholipase A2 action at the single molecule level.Biophysical journal · 2010Article
- Stability of protein-decorated mixed lipid membranes: The interplay of lipid-lipid, lipid-protein, and protein-protein interactions.The Journal of chemical physics · 2009Article
- Effect of membrane composition on antimicrobial peptides aurein 2.2 and 2.3 from Australian southern bell frogs.Biophysical journal · 2009Article
- Activation of phospholipase A2 by ternary model membranes.Biophysical journal · 2008Article
- Molecular basis of phospholipase A2 activity toward phospholipids with sn-1 substitutions.Biophysical journal · 2008Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretory human phospholipase A2 type IIA (PLA2-IIA) catalyzes the hydrolysis of the sn-2 ester bond in glycerolipids to produce fatty acids and lysolipids. The enzyme is coupled to the inflammatory response, and its specificity toward anionic membrane interfaces suggests a role as a bactericidal agent. PLA2-IIA may also target perturbed native cell membranes that expose anionic lipids to the extracellular face. However, anionic lipid contents in native cells appear lower than the threshold levels necessary for activation. By using phosphatidylcholine/phosphatidylglycerol model systems, we show that local enrichment of anionic lipids into fluid domains triggers PLA2-IIA activity. In addition, the compositional range of enzyme activity is shown to be related to the underlying lipid phase diagram. A comparison is done between PLA2-IIA and snake venom PLA2, which in contrast to PLA2-IIA hydrolyzes both anionic and zwitterionic membranes. In general, this work shows that PLA2-IIA activation can be accomplished through local enrichment of anionic lipids into domains, indicating a mechanism for PLA2-IIA to target perturbed native membranes with low global anionic lipid contents. The results also show that the underlying lipid phase diagram, which determines the lipid composition at a local level, can be used to predict PLA2-IIA activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.