ArticlePlant physiology2006
Posttranslational regulation of nitrate reductase strongly affects the levels of free amino acids and nitrate, whereas transcriptional regulation has only minor influence.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 113 citations in OpenAlex.
- Post-Translational Control of Nitrate Reductase Under Elevated COPlants (Basel, Switzerland) · 2026Review
- The Impact of Reduced Nitrogen Fertilizer Application andPlants (Basel, Switzerland) · 2025Article
- Article
- Physiological and transcriptomic analyses of exogenous calcium in boosting nitrogen use efficiency via oxidative and resistance pathways in peanuts.Frontiers in plant science · 2025Article
- Nitrate assimilation pathway is impacted in young tobacco plants overexpressing a constitutively active nitrate reductase or displaying a defective CLCNt2.BMC plant biology · 2024Article
- Long term nitrogen deficiency alters expression of miRNAs and alters nitrogen metabolism and root architecture in Indian dwarf wheat (Triticum sphaerococcum Perc.) genotypes.Scientific reports · 2023Article
- Nitrate reductase enzymes in algaFrontiers in microbiology · 2023Article
- Comprehensive screening of low nitrogen tolerant maize based on multiple traits at the seedling stage.PeerJ · 2022Article
- Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency.Biological research · 2020Review
- Molybdenum-Induced Effects on Nitrogen Metabolism Enzymes and Elemental Profile of Winter Wheat (International journal of molecular sciences · 2019Article
- Expression of novel nitrate reductase genes in the harmful alga, Chattonella subsalsa.Scientific reports · 2018Article
- Mycorrhiza-Triggered Transcriptomic and Metabolomic Networks Impinge on Herbivore Fitness.Plant physiology · 2018Article
- Metabolite profiling and associated gene expression reveal two metabolic shifts during the seed-to-seedling transition in Arabidopsis thaliana.Plant molecular biology · 2017Article
- Nitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms.Plant molecular biology · 2016Article
- Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke.Plant biotechnology journal · 2016Article
- Identification of Nitrogen Use Efficiency Genes in Barley: Searching for QTLs Controlling Complex Physiological Traits.Frontiers in plant science · 2016Article
- A putative role for γ-aminobutyric acid (GABA) in vascular development in pine seedlings.Planta · 2015Article
- S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway.Nature communications · 2014Article
- Impact of the carbon and nitrogen supply on relationships and connectivity between metabolism and biomass in a broad panel of Arabidopsis accessions.Plant physiology · 2013Article
- The mitochondrial folylpolyglutamate synthetase gene is required for nitrogen utilization during early seedling development in arabidopsis.Plant physiology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diurnal variations in nitrate reductase (NR) activity and nitrogen metabolites were examined in wild-type Nicotiana plumbaginifolia and transformants with various degrees of NR deregulation. In the C1 line, NR was only deregulated at the transcriptional level by placing the NR gene under the control of the cauliflower mosaic virus 35S RNA promoter. In the Del8 and S521D lines, NR was additionally deregulated at the posttranslational level either by a deletion mutation in the N-terminal domain or by a mutation of the regulatory phosphorylation site (serine-521). Posttranslational regulation was essential for pronounced diurnal variations in NR activity. Low nitrate content was related to deregulation of NR, whereas the level of total free amino acids was much higher in plants with fully deregulated NR. Abolishing transcriptional and posttranslational regulation (S521D plants) resulted in an increase of glutamine and asparagine by a factor of 9 and 14, respectively, compared with wild type, whereas abolishing transcriptional regulation (C1 plants) only resulted in increases of glutamine and asparagine by factors <2. Among the minor amino acids, isoleucine and threonine, in particular, showed enhanced levels in S521D. Nitrate uptake rates were the same in S521D and wild type as determined with (15)N feeding. Deregulation of NR appears to set the level of certain amino acids, whereas diurnal variations were still determined by light/dark. Generally, deregulation of NR at the transcriptional level did not have much influence on metabolite levels, but additional deregulation at the posttranslational level resulted in profound changes of nitrogen metabolite levels.
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