ArticleThe Plant journal : for cell and molecular biology2006
The 3' untranslated region of a soybean cytosolic glutamine synthetase (GS1) affects transcript stability and protein accumulation in transgenic alfalfa.
Article in The Plant journal : for cell and molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 50 citations in OpenAlex.
- A genome-wide association study reveals molecular mechanism underlying powdery mildew resistance in cucumber.Genome biology · 2024Article
- Artificial selection in breeding extensively enriched a functional allelic variation in TaPHS1 for pre-harvest sprouting resistance in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
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- Overexpression of a glutamine synthetase gene affects growth and development in sorghum.Transgenic research · 2015Article
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- Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme.Frontiers in plant science · 2015Review
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- Trans-Golgi network localized ECHIDNA/Ypt interacting protein complex is required for the secretion of cell wall polysaccharides in Arabidopsis.The Plant cell · 2013Article
- The 5' untranslated region of the soybean cytosolic glutamine synthetase β(1) gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants.Molecular genetics and genomics : MGG · 2012Article
- Comparative analysis of the variable 3' UTR and gene expression of the KIN and KIN-homologous LEA genes in Capsella bursa-pastoris.Plant cell reports · 2012Article
- Evaluation of seed storage protein gene 3'-untranslated regions in enhancing gene expression in transgenic rice seed.Transgenic research · 2012Article
- Glutamine synthetase in legumes: recent advances in enzyme structure and functional genomics.International journal of molecular sciences · 2012Review
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- Repercussion of mesophyll-specific overexpression of a soybean cytosolic glutamine synthetase gene in alfalfa (Plant science : an international journal of experimental plant biology · 2009Article
- Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems.BMC biotechnology · 2008Article
- Characterization of wound-responsive RNA-binding proteins and their splice variants in Arabidopsis.Plant molecular biology · 2008Article
- Nitrogen-dependent posttranscriptional regulation of the ammonium transporter AtAMT1;1.Plant physiology · 2007Article
- Dynamics of a mobile RNA of potato involved in a long-distance signaling pathway.The Plant cell · 2006Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
Higher plants assimilate nitrogen in the form of ammonia through the concerted activity of glutamine synthetase (GS) and glutamate synthase (GOGAT). The GS enzyme is either located in the cytoplasm (GS1) or in the chloroplast (GS2). Glutamine synthetase 1 is regulated in different plants at the transcriptional level and there are some reports of regulation at the level of protein stability. Here we present data that clearly establish that GS1 in plants is also regulated at the level of transcript turnover and at the translational level. Using a Glycine max (soybean) GS1 transgene, with and without its 3' untranslated region (UTR), driven by the constitutive CaMV 35S promoter in Medicago sativa (alfalfa) and Nicotiana tabacum (tobacco), we show that the 3' UTR plays a major role in both transcript turnover and translation repression in both the leaves and the nodules. Our data suggest that the 3' UTR mediated turnover of the transcript is regulated by a nitrogen metabolite or carbon/nitrogen ratios. We also show that the 3' UTR of the gene for the soybean GS1 confers post-transcriptional regulation on a reporter gene. Our dissection of post-transcriptional and translational levels of regulation of GS in plants shows that the situation in plants strongly resembles that in other organisms where GS is regulated at almost all levels. Multistep regulation of GS shows the high priority given by organisms to regulating and ensuring optimal control of nitrogen substrates and preventing overproduction of glutamine and drainage of the glutamate pool.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.