ArticleBMC cancer2006
Nestin expression in the cell lines derived from glioblastoma multiforme.
Article in BMC cancer, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 82 citations in OpenAlex.
- Generation of glioblastoma in mice engrafted with human cytomegalovirus-infected astrocytes.Cancer gene therapy · 2024Article
- Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma Cells.International journal of molecular sciences · 2023Article
- EZH2-Myc driven glioblastoma elicited by cytomegalovirus infection of human astrocytes.Oncogene · 2023Article
- Serotonin limits generation of chromaffin cells during adrenal organ development.Nature communications · 2022Article
- Differences in stem cell marker and osteopontin expression in primary and recurrent glioblastoma.Cancer cell international · 2022Article
- Nestin Regulates Keap1-Nrf2-HO-1-Mediated Antioxidant Responses during Stress and Malignant Hematopoiesis.BioMed research international · 2022Article
- Nestin Is Required for Spindle Assembly and Cell-Cycle Progression in Glioblastoma Cells.Molecular cancer research : MCR · 2021Article
- Targeting PUS7 suppresses tRNA pseudouridylation and glioblastoma tumorigenesis.Nature cancer · 2021Article
- Nestin Expression as a Diagnostic and Prognostic Marker in Colorectal Cancer and Other Tumors.Clinical Medicine Insights. Oncology · 2021Review
- Evaluation of Nestin and EGFR in Patients with Glioblastoma Multiforme in a Public Hospital in Iran.Asian Pacific journal of cancer prevention : APJCP · 2020Article
- Enhanced Antiproliferative Effect of Combined Treatment with Calcitriol and All-International journal of molecular sciences · 2020Article
- Review
- Targeting of replicating CD133 and OCT4/SOX2 expressing glioma stem cells selects a cell population that reinitiates tumors upon release of therapeutic pressure.Scientific reports · 2019Article
- Mimicking brain tumor-vasculature microanatomical architecture via co-culture of brain tumor and endothelial cells in 3D hydrogels.Biomaterials · 2019Article
- Prediction of neuroblastoma cell response to treatment with natural or synthetic retinoids using selected protein biomarkers.PloS one · 2019Article
- Drug Screening of Human GBM Spheroids in Brain Cancer Chip.Scientific reports · 2018Article
- Effects of Sunitinib and Other Kinase Inhibitors on Cells Harboring aInternational journal of molecular sciences · 2018Article
- Cancer stem cells from peritumoral tissue of glioblastoma multiforme: the possible missing link between tumor development and progression.Oncotarget · 2018Article
- Pediatric sarcomas.Oncology letters · 2018Article
- Immunophenotypic characterization of telocyte-like cells in pterygium.Molecular vision · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNestin is a protein belonging to class VI of intermediate filaments that is produced in stem/progenitor cells in the mammalian CNS during development and is consecutively replaced by other intermediate filament proteins (neurofilaments, GFAP). Down-regulated nestin may be re-expressed in the adult organism under certain pathological conditions (brain injury, ischemia, inflammation, neoplastic transformation). Our work focused on a detailed study of the nestin cytoskeleton in cell lines derived from glioblastoma multiforme, because re-expression of nestin together with down-regulation of GFAP has been previously reported in this type of brain tumor.
methodsTwo cell lines were derived from the tumor tissue of patients treated for glioblastoma multiforme. Nestin and other cytoskeletal proteins were visualized using imunocytochemical methods: indirect immunofluorescence and immunogold-labelling.
resultsUsing epifluorescence and confocal microscopy, we described the morphology of nestin-positive intermediate filaments in glioblastoma cells of both primary cultures and the derived cell lines, as well as the reorganization of nestin during mitosis. Our most important result came through transmission electron microscopy and provided clear evidence that nestin is present in the cell nucleus.
conclusionDetailed information concerning the pattern of the nestin cytoskeleton in glioblastoma cell lines and especially the demonstration of nestin in the nucleus represent an important background for further studies of nestin re-expression in relationship to tumor malignancy and invasive potential.
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