ArticleThe Journal of membrane biology2005
Surviving high-intensity field pulses: strategies for improving robustness and performance of electrotransfection and electrofusion.
Article in The Journal of membrane biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Pulsed Electric Field Ablation of Esophageal Malignancies and Mitigating Damage to Smooth Muscle: An In Vitro Study.International journal of molecular sciences · 2023Article
- Substantially Improved Electrofusion Efficiency of Hybridoma Cells: Based on the Combination of Nanosecond and Microsecond Pulses.Bioengineering (Basel, Switzerland) · 2022Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- The effect of temperature conditions during growth on the transformation frequency ofBiochemistry and biophysics reports · 2022Article
- Anhydrous Nucleic Acid Nanoparticles for Storage and Handling at Broad Range of Temperatures.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- The cellular response to plasma membrane disruption for nanomaterial delivery.Nano convergence · 2022Review
- Effects of high-frequency nanosecond pulses on prostate cancer cells.Scientific reports · 2021Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.Cellular and molecular life sciences : CMLS · 2017Article
- A double-pulse approach for electrotransfection.The Journal of membrane biology · 2014Article
- Cell-cell proximity effects in multi-cell electroporation.Biomicrofluidics · 2014Article
- Cell electrofusion using nanosecond electric pulses.Scientific reports · 2013Article
- Dielectric analysis and multi-cell electrofusion of the yeast Pichia pastoris for electrophysiological studies.The Journal of membrane biology · 2012Article
- The systematic study of the electroporation and electrofusion of B16-F1 and CHO cells in isotonic and hypotonic buffer.The Journal of membrane biology · 2012Article
- Use of collagen gel as a three-dimensional in vitro model to study electropermeabilization and gene electrotransfer.The Journal of membrane biology · 2010Article
- Cell-cell electrofusion: optimization of electric field amplitude and hypotonic treatment for mouse melanoma (B16-F1) and Chinese Hamster ovary (CHO) cells.The Journal of membrane biology · 2010Article
- Microfluidic electroporation of tumor and blood cells: observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells.Integrative biology : quantitative biosciences from nano to macro · 2010Article
- Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs.PLoS neglected tropical diseases · 2010Article
- Article
- Parallel single-cell light-induced electroporation and dielectrophoretic manipulation.Lab on a chip · 2009Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrotransfection and electrofusion, both widely used in research and medical applications, still have to face a range of problems, including the existence of electroporation-resistant cell types, cell mortality and also great batch-to-batch variations of the transfection and fusion yields. In the present study, a systematic analysis of the parameters critical for the efficiency and robustness of electromanipulation protocols was performed on five mammalian cell types. Factors examined included the sugar composition of hypotonic pulse media (trehalose, sorbitol or inositol), the kinetics of cell volume changes prior to electropulsing, as well as the growth medium additives used for post-pulse cell cultivation. Whereas the disaccharide trehalose generally allowed regulatory volume decrease (RVD), the monomeric sugar alcohols sorbitol and inositol inhibited RVD or even induced secondary swelling. The different volume responses could be explained by the sugar selectivity of volume-sensitive channels (VSC) in the plasma membrane of all tested cell types. Based on the volumetric data, highest transfection and fusion yields were mostly achieved when the target cells were exposed to hypotonicity for about 2 min prior to electropulsing. Longer hypotonic treatment (10-20 min) decreased the yields of viable transfected and hybrid cells due to (1) the cell size reduction upon RVD (trehalose) or (2) the excessive losses of cytosolic electrolytes through VSC (inositol/sorbitol). Doping the plasma membrane with lipophilic anions prevented both cell shrinkage and ion losses (probably due to VSC inhibition), which in turn resulted in increased transfection and fusion efficiencies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.