ArticlePlant cell reports2006
Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
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- Molecular and cellular dynamics of early embryonic cell divisions in Volvox carteri.The Plant cell · 2022Article
- Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.BMC biology · 2018Article
- Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression.The Plant journal : for cell and molecular biology · 2017Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri.BMC biotechnology · 2015Article
- Stable nuclear transformation of Pandorina morum.BMC biotechnology · 2014Article
- Stable nuclear transformation of Eudorina elegans.BMC biotechnology · 2013Article
- Transient gene expression system established in Porphyra yezoensis is widely applicable in Bangiophycean algae.Marine biotechnology (New York, N.Y.) · 2011Article
- Stable nuclear transformation of Gonium pectorale.BMC biotechnology · 2009Article
- A gender-specific retinoblastoma-related protein in Volvox carteri implies a role for the retinoblastoma protein family in sexual development.The Plant cell · 2008Article
- Identification of the minus-dominance gene ortholog in the mating-type locus of Gonium pectorale.Genetics · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of transgenic organisms is a well-established, versatile course of action in molecular biology. Genetic engineering often requires heterologous, dominant antibiotic resistance genes that have been used as selectable markers in many species. However, as heterologous 5' and 3' flanking sequences often result in very low expression rates, endogenous flanking sequences, especially promoters, are mostly required and are easily obtained in model organisms, but it is much more complicated and time-consuming to get appropriate sequences from less common organisms. In this paper, we show that aminoglycoside 3'-phosphotransferase gene (aphVIII) based constructs with 3' and 5' untranslated flanking sequences (including promoters) from the multicellular green alga Volvox work in the unicellular green alga Chlamydomonas and flanking sequences from Chlamydomonas work in Volvox, at least if a low expression rate is compensated by an enforced high gene dosage. This strategy might be useful for all investigators that intend to transform species in which genomic sequences are not available, but sequences from related organisms exist.
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