ArticleJournal of clinical microbiology2006
Purified excreted-secreted antigens from Trypanosoma cruzi trypomastigotes as tools for diagnosis of Chagas' disease.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.
- Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2020Pooled it
- ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis.BMC infectious diseases · 2010Pooled it
- Chagas disease in Canadian blood donors: 15 years of selective testing.Vox sanguinis · 2026Article
- Stage-InformedMicroorganisms · 2026Article
- Circulating extracellular vesicles in sera of chronic patients as a method for determining active parasitism in Chagas disease.PLoS neglected tropical diseases · 2024Article
- Laboratory diagnosis ofFrontiers in parasitology · 2023Review
- Evidence of likely autochthonous Chagas disease in the southwestern United States: A case series of Trypanosoma cruzi seropositive blood donors.Transfusion · 2022Article
- Worldwide Control and Management of Chagas Disease in a New Era of Globalization: a Close Look at Congenital Trypanosoma cruzi Infection.Clinical microbiology reviews · 2022Review
- Review
- Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease.Pathogens (Basel, Switzerland) · 2021Article
- Mechanisms Associated withLife (Basel, Switzerland) · 2021Review
- All Roads Lead to Cytosol:Frontiers in cellular and infection microbiology · 2021Review
- Prevalence of Chagas Disease Among Family Members of Previously Diagnosed Patients in Los Angeles, California.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Prevalence of Trypanosoma cruzi infection in active military population of The Colombian National Army gathered in five departments.PloS one · 2019Observational
- Prevalence of Chagas Disease in the Latin American-born Population of Los Angeles.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells.Journal of clinical microbiology · 2017Article
- A Brief View of the Surface Membrane Proteins fromJournal of parasitology research · 2017Review
- Serological Diagnosis of Chronic Chagas Disease: Is It Time for a Change?Journal of clinical microbiology · 2016Article
- Insight into the Exoproteome of the Tissue-Derived Trypomastigote form ofFrontiers in chemistry · 2016Article
- Apoptotic-like Leishmania exploit the host's autophagy machinery to reduce T-cell-mediated parasite elimination.Autophagy · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is currently no "gold standard" test for the diagnosis of late-stage Chagas' disease. As a result, protection of the blood supply in areas where Chagas' disease is endemic remains problematic. A panel of 709 serum samples from subjects with confirmed Chagas' disease (n = 195), healthy controls (n = 400), and patients with other parasitic diseases (n = 114) was used to assess enzyme-linked immunosorbent assays (ELISAs) based on a concentrated extract of excretory-secretory antigens from either Brazil or Tulahuen strain Trypanosoma cruzi trypomastigotes (total trypomastigote excretory-secretory antigens [TESAs]). The total TESA-based assays had excellent overall sensitivity (100%) and specificity (>94%), except for cross-reactivity with Leishmania-infected sera. In an attempt to increase the specificity of the assay, immunoaffinity chromatography was used to purify the TESA proteins (TESA(IA) proteins). By Western blotting, a series of polypeptide bands with molecular masses ranging from 60 to 220 kDa were recognized by pooled sera positive for Chagas' disease. An ELISA based on TESA(IA) proteins had a slightly lower sensitivity (98.6%) but an improved specificity (100%) compared to the sensitivity and specificity of the total TESA protein-based ELISAs. A 60-kDa polypeptide was identified as a major contributor to the cross-reactivity with Leishmania. These data suggest the need for field validation studies of TESA- and TESA(IA)-based assays in regions where Chagas' disease is endemic.
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