ArticleThe Journal of physiology2006
Extracellular guanosine-5'-triphosphate modulates myogenesis via intermediate Ca(2+)-activated K+ currents in C2C12 mouse cells.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Challenges in the Therapeutic Targeting of KCa Channels: From Basic Physiology to Clinical Applications.International journal of molecular sciences · 2024Review
- CaInternational journal of molecular sciences · 2023Review
- Ion Channels and Transporters in Muscle Cell Differentiation.International journal of molecular sciences · 2021Review
- Raising the Guanosine-Based Molecules as Regulators of Excitable Tissues by the Exosomal-Vehiculated Signaling.Frontiers in pharmacology · 2021Article
- Unfolding New Roles for Guanine-Based Purines and Their Metabolizing Enzymes in Cancer and Aging Disorders.Frontiers in pharmacology · 2021Article
- Guanosine-Based Nucleotides, the Sons of a Lesser God in the Purinergic Signal Scenario of Excitable Tissues.International journal of molecular sciences · 2020Review
- The LRRC8/VRAC anion channel facilitates myogenic differentiation of murine myoblasts by promoting membrane hyperpolarization.The Journal of biological chemistry · 2019Article
- Extracellular Guanosine 5'-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine.Frontiers in pharmacology · 2018Article
- Low Intensity Exercise Training Improves Skeletal Muscle Regeneration Potential.Frontiers in physiology · 2015Article
- New insights into the relationship between mIGF-1-induced hypertrophy and Ca2+ handling in differentiated satellite cells.PloS one · 2014Article
- Purinergic signalling in the musculoskeletal system.Purinergic signalling · 2013Review
- Transcriptional profile of GTP-mediated differentiation of C2C12 skeletal muscle cells.Purinergic signalling · 2012Article
- Signal transduction events induced by extracellular guanosine 5' triphosphate in excitable cells.Purinergic signalling · 2006Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we investigated the role of extracellular 5'-guanosine-triphosphate (GTP) on early phases of skeletal muscle differentiation using the widely used C2C12 mouse cells as a myogenic model. We show that extracellular GTP binding to specific sites activates a metabotropic cascade that leads to a transient intracellular Ca2+ mobilization, consequent activation of the intermediate Ca(2+)-activated K+ channels (IK(Ca)), and hyperpolarization of the plasma membrane. We further show that in differentiating C2C12 myoblasts GTP induces a proliferative boost, and increases the number of cells positive for the myosin heavy chain (MyHC) proteins. These effects were shown to be mediated by the IK(Ca) channel-dependent hyperpolarization, as evidenced by their disappearance when myoblasts were incubated with the IK(Ca) channel inhibitor charybdotoxin. These data give new insights into nucleotide purinergic signalling pathways, and address the role of the GTP-dependent IK(Ca) channel activation and hyperpolarization in myogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.