ArticleMolecular and cellular biology1991
Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Human gene therapy comes of age.Nature · 1992Trial
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- Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency.Nucleic acids research · 2021Article
- Experimental System to Study Instability of (CGG)Methods in molecular biology (Clifton, N.J.) · 2020Article
- Bi-allelic Homology-Directed Repair with Helper-Dependent Adenoviruses.Molecular therapy. Methods & clinical development · 2019Article
- FANCD2 binding identifies conserved fragile sites at large transcribed genes in avian cells.Nucleic acids research · 2018Article
- New Plasmids forFrontiers in microbiology · 2018Article
- Sequential and counter-selectable cassettes for fission yeast.BMC biotechnology · 2016Article
- Identification of potential drug targets implicated in Parkinson's disease from human genome: insights of using fused domains in hypothetical proteins as probes.ISRN neurology · 2011Article
- Circadian rhythms in Neurospora crassa: dynamics of the clock component frequency visualized using a fluorescent reporter.Fungal genetics and biology : FG & B · 2010Article
- Genetically engineered T cells to target EGFRvIII expressing glioblastoma.Journal of neuro-oncology · 2009Article
- Redirecting specificity of T-cell populations for CD19 using the Sleeping Beauty system.Cancer research · 2008Article
- Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage phiBT1.Nucleic acids research · 2008Article
- Adoptive cellular immunotherapy for childhood malignancies.Bone marrow transplantation · 2008Review
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- Iterative in vivo assembly of large and complex transgenes by combining the activities of phiC31 integrase and Cre recombinase.Nucleic acids research · 2005Article
- The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer.Chromosoma · 2005Review
- Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module.Eukaryotic cell · 2005Article
- Rearranging the centromere of the human Y chromosome with phiC31 integrase.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.