ArticleThe EMBO journal1985
Probing an adhesion mutant of Dictyostelium discoideum with cDNA clones and monoclonal antibodies indicates a specific defect in the contact site A glycoprotein.
Article in The EMBO journal, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- The Dynamics of Aerotaxis in a Simple Eukaryotic Model.Frontiers in cell and developmental biology · 2021Article
- Cellular allorecognition and its roles in Dictyostelium development and social evolution.The International journal of developmental biology · 2019Review
- The Dictyostelium discoideum GPHR ortholog is an endoplasmic reticulum and Golgi protein with roles during development.Eukaryotic cell · 2015Article
- The Dictyostelium discoideum RACK1 orthologue has roles in growth and development.Cell communication and signaling : CCS · 2014Article
- Polymorphic members of the lag gene family mediate kin discrimination in Dictyostelium.Current biology : CB · 2009Article
- The Dictyostelium LIM domain-containing protein LIM2 is essential for proper chemotaxis and morphogenesis.Molecular biology of the cell · 2000Article
- Phototactic migration of Dictyostelium cells is linked to a new type of gelsolin-related protein.Molecular biology of the cell · 1999Article
- Functional promiscuity of gene regulation by serpentine receptors in Dictyostelium discoideum.Molecular and cellular biology · 1998Article
- The hybrid histidine kinase DokA is part of the osmotic response system of Dictyostelium.The EMBO journal · 1996Article
- Replacement of the phospholipid-anchor in the contact site A glycoprotein of D. discoideum by a transmembrane region does not impede cell adhesion but reduces residence time on the cell surface.The Journal of cell biology · 1994Article
- The Ca(2+)-binding domains in non-muscle type alpha-actinin: biochemical and genetic analysis.The Journal of cell biology · 1993Article
- Constitutive overexpression of the contact site A glycoprotein enables growth-phase cells of Dictyostelium discoideum to aggregate.The EMBO journal · 1990Article
- Membrane-enclosed crystals in Dictyostelium discoideum cells, consisting of developmentally regulated proteins with sequence similarities to known esterases.The Journal of cell biology · 1990Article
- A pair of tandemly repeated genes code for gp24, a putative adhesion protein of Dictyostelium discoideum.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Adhesion molecules and animal development.Experientia · 1990Review
- The contact site A glycoprotein of Dictyostelium discoideum carries a phospholipid anchor of a novel type.The EMBO journal · 1989Article
- Genetics of early Dictyostelium discoideum development.Microbiological reviews · 1988Review
- Cell surface oligosaccharides participate in cohesion during aggregation of Dictyostelium discoideum.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Caffeine and heat shock induce adenylate cyclase in Dictyostelium discoideum.The EMBO journal · 1986Article
- Studies on the transcription, translation, and structure of alpha-actinin in Dictyostelium discoideum.The Journal of cell biology · 1986Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of developmentally regulated membrane proteins of aggregating cells of Dictyostelium discoideum is subject to several control mechanisms. One of them involves periodic cyclic-AMP pulses as signals for gene expression. To increase the probability of selecting mutants specifically defective in the contact site A (csA) glycoprotein, one of the characteristic proteins of aggregating cells, we have bypassed the requirement for both cyclic-AMP pulses and another control element by two runs of mutagenesis. A ;double bypass' mutant, HG592, was obtained which aggregated in nutrient medium where wild-type did not develop. Mutants defective in expression of the csA-glycoprotein were selected from HG592 by fluorescence-activated cell sorting and colony immunoblotting using a monoclonal antibody specific for that protein. One among 51 csA-negative mutants, HG693, specifically lacked the capability of forming EDTA-stable intercellular contacts. It acquired chemotactic responsiveness and developed into fruiting bodies. Expression of the transcripts for eight developmentally regulated proteins was determined in HG693. Seven of the RNA species were normally expressed; they were recognized by cDNA clones which had been produced from poly(A) RNA isolated from membrane-bound polysomes. The single RNA species which was not substantially expressed in HG693 was recognized by a cDNA clone that was obtained by screening a lambdagt11 library with an antibody specific for the csA-glycoprotein. When probing RNA from wild-type cells, this clone hybridized with a single developmentally regulated RNA species of 1.9 kb whose expression was strongly enhanced by cyclic-AMP pulses. Appearance of this RNA coincided with the expression of the csA-glycoprotein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.