ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
Utilization of the three high-throughput SNP genotyping methods, the GOOD assay, Amplifluor and TaqMan, in diploid and polyploid plants.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
- Developing and deploying an efficient genotyping workflow for accelerating maize improvement in developing countries.Gates open research · 2022Article
- Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET.Frontiers in plant science · 2021Article
- An extended KASP-SNP resource for molecular breeding in Chinese cabbage(Brassica rapa L. ssp. pekinensis).PloS one · 2020Article
- Review
- A SimpleProbeJournal of clinical laboratory analysis · 2019Article
- Two sugar beet chitinase genes,PeerJ · 2018Article
- Genotyping-by-sequencing application on diploid rose and a resulting high-density SNP-based consensus map.Horticulture research · 2018Article
- Advantages of Amplifluor-like SNP markers over KASP in plant genotyping.BMC plant biology · 2017Article
- Expression Level of theFrontiers in plant science · 2016Article
- Efficient SNP Discovery by Combining Microarray and Lab-on-a-Chip Data for Animal Breeding and Selection.Microarrays (Basel, Switzerland) · 2015Review
- Digital genotyping of sorghum - a diverse plant species with a large repeat-rich genome.BMC genomics · 2013Article
- SNP Discovery through Next-Generation Sequencing and Its Applications.International journal of plant genomics · 2012Article
- A unified approach to the estimation and interpretation of resistance costs in plants.Heredity · 2011Review
- Multiplex single nucleotide polymorphism (SNP) assay for detection of soybean mosaic virus resistance genes in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Model SNP development for complex genomes based on hexaploid oat using high-throughput 454 sequencing technology.BMC genomics · 2011Article
- A high-throughput method for the detection of homologous gene deletions in hexaploid wheat.BMC plant biology · 2010Article
- Identification of single nucleotide polymorphisms and analysis of linkage disequilibrium in sunflower elite inbred lines using the candidate gene approach.BMC plant biology · 2008Article
- Identification, validation and survey of a single nucleotide polymorphism (SNP) associated with pungency in Capsicum spp.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007Article
- A comparison of simple sequence repeat and single nucleotide polymorphism marker technologies for the genotypic analysis of maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007Article
- Protocol: An improved high-throughput method for generating tissue samples in 96-well format for plant genotyping (Ice-Cap 2.0).Plant methods · 2007Article
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10 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The application of high-throughput SNP genotyping is a great challenge for many research projects in the plant genetics domain. The GOOD assay for mass spectrometry, Amplifluor and TaqMan are three methods that rely on different principles for allele discrimination and detection, specifically, primer extension, allele-specific PCR and hybridization, respectively. First, with the goal of assessing allele frequencies by means of SNP genotyping, we compared these methods on a set of three SNPs present in the herbicide resistance genes CSR, AXR1 and IXR1 of Arabidopsis thaliana. In this comparison, we obtained the best results with TaqMan based on PCR specificity, flexibility in primer design and success rate. We also used mass spectrometry for genotyping polyploid species. Finally, a combination of the three methods was used for medium- to high-throughput genotyping in a number of different plant species. Here, we show that all three genotyping technologies are successful in discriminating alleles in various plant species and discuss the factors that must be considered in assessing which method to use for a given application.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.