Evidence map›Paper›PMID 16453000›Full record

ArticleLeukemia2006

Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.

J Ratajczak, K Miekus, M Kucia, J Zhang, R Reca, P Dvorak, M Z Ratajczak

Abstract read
PubMed Publisher
In one paragraph

Article in Leukemia, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 904 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
904citing papers in PubMed, 1 pooled it
9.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

904 citing papers in PubMed, 1 synthesis or guideline pooled it, 1,623 citations in OpenAlex.

  1. Pooled it
  2. Trial
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  4. Trial
  5. Review
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  7. Article
  8. Article
  9. Research progress on biomarkers of traumatic brain injury.Animal models and experimental medicine · 2026
    Review
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  12. Article
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  17. Rethinking Extracellular Vesicle Signaling.Advanced materials (Deerfield Beach, Fla.) · 2026
    Article
  18. Article
  19. Review
  20. Article

844 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 3 countries.

J RatajczakStem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
K Miekus
M Kucia
J Zhang
R Reca
P Dvorak
M Z Ratajczak
University of Louisville · USCzech Academy of Sciences · CZ

Funding

The CXCR4-SDF-1 Axis in Metastatic RhabdomyosarcomaR01CA106281 · NCI · UNIVERSITY OF LOUISVILLE · PI RATAJCZAK, MARIUSZ Z · 2005 to 2009
$1.3M
NCI NIH HHS R01 CA106281-01
6 · The paper itself

Abstract

Membrane-derived vesicles (MV) are released from the surface of activated eucaryotic cells and exert pleiotropic effects on surrounding cells. Since the maintenance of pluripotency and undifferentiated propagation of embryonic stem (ES) cells in vitro requires tight cell to cell contacts and effective intercellular signaling, we hypothesize that MV derived from ES cells (ES-MV) express stem cell-specific molecules that may also support self-renewal and expansion of adult stem cells. To address this hypothesis, we employed expansion of hematopoietic progenitor cells (HPC) as a model. We found that ES-MV (10 microg/ml) isolated from murine ES cells (ES-D3) in serum-free cultures significantly (i) enhanced survival and improved expansion of murine HPC, (ii) upregulated the expression of early pluripotent (Oct-4, Nanog and Rex-1) and early hematopoietic stem cells (Scl, HoxB4 and GATA 2) markers in these cells, and (iii) induced phosphorylation of MAPK p42/44 and serine-threonine kinase AKT. Furthermore, molecular analysis revealed that ES-MV express Wnt-3 protein and are selectively highly enriched in mRNA for several pluripotent transcription factors as compared to parental ES cells. More important, this mRNA could be delivered by ES-MV to target cells and translated into the corresponding proteins. The biological effects of ES-MV were inhibited after heat inactivation or pretreatment with RNAse, indicating a major involvement of protein and mRNA components of ES-MV in the observed phenomena. We postulate that ES-MV may efficiently expand HPC by stimulating them with ES-MV expressed ligands (e.g., Wnt-3) as well as increase their pluripotency after horizontal transfer of ES-derived mRNA.

Indexed as

AnimalsAntigens, LyBiological TransportCell CommunicationCell LineCell Membrane StructuresCell SurvivalCytoplasmEmbryo, MammalianHematopoietic Stem CellsHumansIn Vitro TechniquesMembrane ProteinsMiceMitogen-Activated Protein KinasesOctamer Transcription Factor-3Antigens, LyLy6a protein, mouseMembrane ProteinsMitogen-Activated Protein KinasesOctamer Transcription Factor-3Proto-Oncogene Proteins c-aktRNA, MessengerWnt3 ProteinWNT3 protein, humanWnt3 protein, mouseWnt Proteins

Identifiers

PMID16453000
OpenAlexW1967367827

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.